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A gradual disruption of tight side–chain packing: 2D 1H–NMR characterization of acid–induced unfolding of CHABII

Nature Structural Biology volume 6, pages 129–134 (1999)Cite this article

Abstract

Little is known about the mechanism of the transition between native proteins and partially folded intermediates. Complete assignments of 2D 1H–NOESY spectra of CHABII at 5 °C, pH 6.3, 5.5, 4.6 and 4.0, reveal that lowering of pH results in an extensive but gradual disappearance of NOEs, implying a gradual disruption of tight side–chain packing. Moreover, a tertiary packing core is identified at 5 °C and pH 4.0, characterized by persistent long–range NOEs. Thus, we suggest that severe disruption of tight side–chain packing of CHABII can occur at a stage where its secondary structure and tertiary topology remain highly native–like.

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Figure 1: Schematic representation of CHABII.
Figure 2: a, Far-UV CD spectra of CHABII at 5 °C and and pH 6.3, 5.5, 4.6, 4.0 and 1.5.
Figure 3: NH regions of 2D 1H NOESY spectra of CHABII (600 MHz, mixing time of 150 ms) at 5°C.
Figure 4: a–d, Schematic representation of NOE data used for identification of secondary structure.
Figure 5: Numbers or NOE connectivities per residue for intraresidual NOEs (open bars), sequential NOEs (diagonally hatched bars) medium NOEs (horizontally hatched bars), and long range NOEs (filled bars).

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Acknowledgements

We thank Z.Y. Peng, Department of Biochemistry, University of Connecticut Health Centre, for helpful discussion, and T. Sprules at the Biotechnology Research Institute for reading the manuscript.

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  1. Jianxing Song

    Present address: Biotechnology Research Institute, National Research Council, 6100 Royalmount Avenue, Montreal, Quebec, H4P 2R2, Canada

Authors and Affiliations

  1. Département d'Ingénierie et d'Etudes des Protéines, CEA, Gif–sur–Yvette Cedex, 91191, Saclay, France

    Jianxing Song, Nadège Jamin, Bernard Gilquin, Claudio Vita & André Ménez

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Correspondence to Jianxing Song.

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Song, J., Jamin, N., Gilquin, B. et al. A gradual disruption of tight side–chain packing: 2D 1H–NMR characterization of acid–induced unfolding of CHABII . Nat Struct Mol Biol 6, 129–134 (1999). https://doi.org/10.1038/5815

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