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Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain

Nature Structural Biology volume 5, pages 55–59 (1998)Cite this article

Abstract

A novel off-resonance rotating-frame 15N NMR spin relaxation experiment is used to characterize conformational fluctuations with correlation times between 32 and 175 μs in the third fibronectin type III domain of human tenascin-C. Conformational fluctuations of contiguous regions of the β-sandwich structure of the type III domain may represent collective motions, such as transient twisting or breathing of the β-sheets. Flexibility of the loop containing the Arg-Gly-Asp (RGD) tripeptide may affect the accessibility of this motif in protein-protein interactions.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York, 10032, USA

    Mikael Akke & Arthur G. Palmer III

  2. Pharmacology Research & Development, Genentech, Inc., South San Francisco, California, 94080, USA

    Jun Liu

  3. NMR Structural Biology Facility, Wadsworth Center, New York State Department of Health, Albany, NY, 12201, USA

    John Cavanagh

  4. Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, 27710, USA

    Harold P. Erickson

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  4. Harold P. Erickson
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  5. Arthur G. Palmer III
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Akke, M., Liu, J., Cavanagh, J. et al. Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain. Nat Struct Mol Biol 5, 55–59 (1998). https://doi.org/10.1038/nsb0198-55

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