Abstract
The crystal structure of the RNA dodecamer 5′-GGACUUUGGUCC-3′ has been determined from X-ray diffraction data to 2.6 Å resolution. This oligomer forms an asymmetric double helix in the crystal. Four consecutive non-Watson-Crick base-pairs are formed in the middle of the duplex including the first intrahelical U-U (or T-T) pairs observed in an oligonucleotide crystal structure. Two different conformations of U-U pairs are observed in the context of the surrounding sequence. One of these pairs is highly twisted, allowing a bound water to bridge across strands in the major groove. The crystal packing illustrates a new form of RNA helix-helix interaction.
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Baeyens, K., De Bondt, H. & Holbrook, S. Structure of an RNA double helix including uracil-uracil base pairs in an internal loop. Nat Struct Mol Biol 2, 56–62 (1995). https://doi.org/10.1038/nsb0195-56
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DOI: https://doi.org/10.1038/nsb0195-56
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