Determination of the pKa values of the γ-phosphate in p21ras mutants provides the first experimental evidence for substrate-assisted catalysis in the intrinsic GTPase reaction.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Bourne, H.R., Sanders, D.A. & McCormick, F. Nature 348, 125–132 (1990).
Bourne, H.R., Sanders, D.A. & McCormick, F. Nature 349, 117–127 (1991).
Schweins, T. et al. Nature struct. Biol. 2, 36–44(1995).
Eccleston, J.F., Webb, M.R., Ash, D.E. & Reed, G.H. J. biol. Chem. 256, 10774–10777 (1981).
Feuerstein, J., Goody, R.S. & Webb, M.R. J. biol. Chem. 264, 6188–6190 (1989).
Pai, E.F. et al. EMBO J. 9 2351–2359 (1990).
Der, C.J., Finkel, T. & Cooper, G.M. Cell 44, 167–176 (1986).
John, J., Frech, M. & Wittinghofer, A. J. biol. Chem. 263, 11792–11799 (1988).
Krengel, U. et al. Cell 62, 539–548 (1990).
Chung, H.H., Benson, D.R. & Schultz, P.G. Science 259, 806–809 (1993).
Privé, G.G. et al. Proc. natn. Acad. Sci. U.S.A. 89, 3649–3653 (1992).
Frech, M. et al. Biochemistry 33, 3237–3244 (1994).
Cool, R.H. & Parmeggiani, A. Biochemistry 30,362–366 (1991).
Kjeldgaard, M. & Nyborg, J. J. molec. Biol. 223, 721–742 (1992).
Berchtold, H. et al. Nature 365, 126–132 (1993).
Jacquet, E. & Parmeggiani, A. EMBO J. 7, 2861–2867 (1988).
Noel, J.P., Hamm, H.E. & Sigler, P.B. Nature 366, 654–663 (1993).
Lambright, D.G., Noel, J.P., Hamm, H.E. & Sigler, P.B. Nature 369, 621–628 (1994).
Coleman, D.E. et al. Science 265, 1405–1412 (1994).
Abood, M.E., Hurley, J.B., Pappone, M.-C., Bourne, H.R. & Stryer, L. J. biol. Chem. 257, 10540–10543 (1982).
VanDop, C., Tsubokawa, M., Bourne, H.R. & Ramachandran, J. J. biol. Chem. 259, 696–698 (1984).
Graziano, M.P. & Gilman, A.G. J. biol. Chem. 264, 15475–15482 (1989).
Jurnak, F., Heffron, S., Schick, B. & Delaria, K. Biochim. biophys. Acta 1050, 209–214 (1990).
Kjeldgaard, M., Nissen, P., Thirup, S. & Nyborg, J. Structure 1, 35–50 (1993).
Förster, C., Limmer, S., Zeidler, W. & Sprinzl, M. Proc. natn. Acad. Sci. U.S.A. 91, 4254–4257 (1994).
Freissmuth, M. & Gilman, A.G. J. biol. Chem. 264, 21907–21914 (1989).
Kleuss, C., Raw, A.S., Lee, E., Sprang, S.R. & Gilman, A.G. Proc. natn. Acad. Sci. U.S.A. 91, 9828–9831 (1994).
Chabre, M. Trends Biochem. Sci. 15, 6–10 (1990).
Sondek, J., Lambright, D.G., Noel, J.P., Hamm, H.E. & Sigler, P.B. Nature 372, 276–279 (1994).
Kahn, R.A. J. biol. Chem. 266, 15595–15597 (1991).
Mesters, J.R., de Graaf, J.M. & Kraal, B. FEBS Letts. 321, 149–152 (1993).
Langen, R., Schweins, T. & Warshel, A. Biochemistry 31, 8691–8696 (1992).
Schweins, T., Langen, R. & Warshel, A. Nature struct. Biol. 1, 476–484 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hilgenfeld, R. How do the GTPases really work?. Nat Struct Mol Biol 2, 3–6 (1995). https://doi.org/10.1038/nsb0195-3
Issue Date:
DOI: https://doi.org/10.1038/nsb0195-3
This article is cited by
-
GTPases of the Translation Apparatus
Molecular Biology (2005)