Dental caries is caused by one of the most ubiquitous bacterial infections of humans. In many countries such as Brazil and China, this disease is reaching epidemic proportions, and it is clear that a more effective public-health measure to combat dental caries is needed, because disadvantaged children are the most severely affected. One of the main groups of oral microorganisms, the mutans streptococci, has been associated with the aetiology of dental caries, and preclinical studies of immunological interventions have shown the feasibility of interfering with this disease. Moreover, clinical trials have indicated that a mucosal immune response to a crucial antigen(s) of mutans streptococci can influence the pathogenesis of dental caries. Evidence that this antigen(s) is appropriate for use in a vaccine against dental caries, as well as evidence for an appropriate target population of individuals and a logical time of administration, has now emerged.
Oral health is a substantial component of general health, well-being and quality of life. Unfortunately, dental caries (the soft, decayed areas in teeth that, if allowed to progress, can lead to tooth destruction) remains a chronic infectious disease that not only is widespread in industrialized countries but also is becoming prevalent in the world's poorest developing countries. The World Health Organization (WHO) affirms that dental caries qualifies as a major public-health problem, owing to its high prevalence in all regions of the world, with the greatest burden of disease being on disadvantaged and socially marginalized populations1.
The World Oral Health Report 2003 (Ref. 1), published by the WHO, indicates that dental caries is a major health problem in most industrialized countries, affecting 60–90% of school children and most adults. The average number of decayed, missing and filled permanent teeth for individuals of 12 years of age is 3.5 in the Americas, 2.4 in the western Pacific, 2.0 in Europe, 2.0 in the eastern Mediterranean, and 1.5 in Southeast Asia and Africa. It is expected that the prevalence of dental caries will increase in Africa as a result of growing consumption of sugar and inadequate exposure to fluoride. In the United States, 98% of individuals of 40–44 years of age have experienced carious infection (with an average of 44 carious surfaces per individual). Worldwide, there are staggering levels of edentulism (see Glossary), for which dental caries is a major contributor. For example, the following are rates of edentulism for those aged 65 years or older: in Bosnia and Herzegovina, 78%; Albania, 69%; Canada, 58%; Great Britain, 46%; Finland, 41%; Denmark, 27%; and the United States, 26%.
In the United States, dental caries is the most common childhood disease. Furthermore, dental care for children has been identified as the most prevalent unmet health need. The Surgeon General's report Oral Health in America2, which was published in 2000, documented the considerable disparities in oral health among those living in the United States. Of the total number of carious surfaces found in children, 80% are found in only 20–25% of children (that is, in ∼20 million children, who are mainly from African-American, Hispanic, American-Indian, Alaska-Native and low-income families)105. In addition, early childhood dental caries (also known as baby-bottle tooth decay) occurs in 70–90% of very young children in certain socio-economically defined populations105.
It is estimated that, in 2004, Americans spent US$75 billion on oral health care. This is ∼5% of the total health-care expenditure in the United States. Although this sum includes expenditure for other dental treatments, most of it went towards treatment of dental caries and its sequelae. Many other countries that are more susceptible to the ravages of dental caries are less able to implement practices to control the disease — such as use of restorations (that is, fillings), treatment with fluoride, restriction of dietary sucrose and use of good dental-hygiene practices — so the dental-caries epidemic continues to grow.
Molecular pathogenesis of dental caries
Dental caries results from the dissolution of minerals that are present in the enamel and dentine of teeth by organic acids (such as lactic acid). These acids are metabolic end-products that are excreted by certain microbiota in the dental biofilm, most notably by mutans streptococci (a group of Streptococcus spp. that is found in the oral cavity and is associated with dental caries in humans and other species)3,4. In 1924, Clark5 isolated streptococci from a human carious tooth lesion and called the bacteria Streptococcus mutans. More recently, similar mutans streptococci have been classified into several species, with Streptococcus sobrinus and S. mutans3,4,6,7 being the most often isolated from humans and the most often associated with the aetiology of dental caries8,9. The presence of dental-caries-associated streptococci in the mouth of nearly all adults indicates that dental caries is probably the most ubiquitous bacterial infectious disease of humans10. Although other oral microorganisms can be cariogenic, mutans streptococci have unique biochemical features that make them efficient at accumulating and producing carious surfaces; therefore, they are a good target for therapies aimed at the prevention of dental caries. The characteristics that make mutans streptococci particularly efficient at causing dental caries include production of large amounts of lactic acid at a rapid rate and tolerance to extremes of sugar concentration, ionic strength and pH3. Dental caries can be initiated and transmitted to others by infection with mutans streptococci, especially in the presence of dietary sucrose, which favours the accumulation of mutans streptococci (discussed later). Animal studies3,11,12 and clinical trials13,14,15 have shown that the incidence of dental caries can be reduced by the administration of antibiotics or antibodies that target mutans streptococci, further implicating these microorganisms in the pathogenesis of dental caries.
The molecular pathogenesis of mutans-streptococcal-associated dental caries can be thought of as occurring in three phases. The first phase involves the initial attachment of the microorganism to the dental pellicle16. This is mediated by an adhesin from mutans streptococci that is known as antigen I/II (also known as P1 and PAc)17,18,19,20 (Fig. 1a). The second phase, which is known as accumulation, depends on the presence of sucrose, as well as glucosyltransferases (GTFs) and glucan-binding proteins (GBPs) from mutans streptococci (Fig. 1b). After cleaving sucrose into its component saccharides (glucose and fructose), mutans streptococci GTFs synthesize glucans that have various α-1,3- and α-1,6-linkages and different solubilities in water. In the third phase, the multivalent glucans that have been produced interact with GBPs and with the glucan-binding domain of GTFs, both of which are present at the surface of mutans streptococci. The aggregation and the multiplication of these bacteria result in the accumulation of biofilms that are known as dental plaques, which are composed of masses of mutans streptococci. When these accumulations are of sufficient magnitude and when sugars (including sucrose and glucose) that are substrates for these bacteria are available, large amounts of lactic acid are produced21, causing enamel dissolution and carious lesions (Fig. 1c).
History of vaccination. The principle of immunization against dental caries was first shown by Bowen12. Although he did not measure immunological parameters, Bowen showed that monkeys that were immunized intravenously with S. mutans developed little carious disease. Rodent dentition is anatomically less similar to human dentition than non-human-primate dentition; however, the composition of all three is similar, and both rodents and non-human primates are excellent models for studies of dental caries. The principle of vaccination against dental caries was then extended to include the involvement of mucosal immunity. This stemmed from a study in which rats were immunized subcutaneously in the vicinity of the salivary glands with mutans streptococci11. This immunization induced salivary secretory IgA responses22, the levels of which directly correlated with a reduction in the number of bacteria recovered after experimental mutans streptococcal infection, as well as a reduction in the development of subsequent disease11. Further studies also used local immunization of rats to induce salivary secretory IgA, which led to reductions in the number of mutans streptococci and the extent of dental caries23,24. In these studies, serum IgG specific for mutans streptococci was also present.
Michalek and co-workers25 were the first to show that induction of immunity by a different mucosal route (the feeding of bacteria) was sufficient to elicit a protective salivary secretory IgA response in rats without the induction of detectable specific serum IgG. Limited investigations in non-human primates indicated a significant correlation between the concentration of serum and gingival crevicular fluid IgG (but not salivary IgA) specific for bacterial antigen and the reduction in dental caries after parenteral immunization with a mutans streptococcal protein antigen26,27. Mestecky and colleagues28 then showed that feeding humans mutans streptococci in gelatin capsules elicited specific salivary secretory IgA. These experiments helped to initiate the concept of a common mucosal immune system, because secretory IgA concentrations were also increased in tears. Intraductal immunization (into Stensen's duct) of non-human primates with intact mutans streptococci also induced salivary IgA29, and this was associated with a marked reduction in dental caries caused by infection with mutans streptococci30.
For dental caries in which mutans streptococci seem to be the main causative agent, these early studies therefore indicated that, under controlled conditions, the host immune response to S. mutans could interfere with the development of dental caries. This association between the mucosal immune response and protection against cariogenic bacterial infection has been considerably strengthened by subsequent experiments31,32,33.
Mechanism of action. Mucosal immunization with mutans streptococcal antigens at inductive sites, including gut-associated lymphoid tissue (GALT) and nasopharynx-associated lymphoid tissue (NALT), results in the migration of antigen-specific IgA-producing B cells to effector organs, such as the salivary glands. This is followed by the differentiation and maturation of these B cells and the secretion of IgA in the lamina propria, where it crosses the effector-tissue ducts into the saliva34. The three main types of mutans streptococcal antigen that are involved in dental-caries pathogenesis and for which specific secretory IgAs have been found are antigen I/II, GTFs and GBPs. Although T cells present in the peripheral-blood lymphocyte population of individuals with dental caries have been shown to respond to mutans streptococcal GTF and antigen I/II (Ref. 35), it is not clear whether T cells have a role in protection, other than by providing help for the synthesis of IgA by B cells.
Several lines of evidence indicate that the presence of specific antibody might modify the course of infection and disease with cariogenic mutans streptococci32. For example, antibody specific for GTF, when incubated in vitro with sucrose and growing cultures of mutans streptococci, markedly reduced the amount of plaque formed on hard surfaces36. Epidemiological evidence for the effects of specific antibodies is more problematic to obtain because of uncontrolled variables such as diet, amount of fluoride exposure, type of concomitant medication and length of association with the cariogenic microorganisms.
In theory, several phases of mutans streptococcal infection are susceptible to immune intervention. Microorganisms in the saliva could be cleared from the oral cavity by antibody-mediated aggregation before these organisms are able to colonize the teeth. Antibody could also block bacterial-surface receptors that are required for colonization or accumulation of bacteria, or it could inactivate the enzymes that are responsible for glucan formation or modify metabolically important functions of these and other enzymes. The presence of S. mutans-specific IgG in the gingival crevicular fluid37 or of S. mutans-specific secretory IgA in the saliva13,14 has been associated with short-term inhibition of colonization of the teeth by mutans streptococci that are indigenous or have been introduced. These studies have indicated that a functioning immune system is crucial for oral health and that antibody of the appropriate specificity could influence the course of dental caries, particularly if specific antibody were present at the initiation of cariogenic infectious challenge.
Selection of vaccine antigens
There are several types of vaccine against dental caries in development, and these differ in terms of their target antigen(s). Several purified antigens from mutans streptococci have been shown to induce protective immunity in experimental models of dental caries, and these antigens form a basis for vaccine development (Table 1). Importantly, each of these vaccine candidates is known to be involved in the molecular pathogenesis of dental caries (Fig. 1).
Surface adhesin. Russell and Lehner17 were the first to describe the protein known as antigen I/II, which is found in supernatant from S. mutans cultures and at the surface of S. mutans. This 190 kDa adhesin has since been cloned and sequenced18,38. The cellular function of antigen I/II remains unclear, but regions of the antigen that bind human salivary proteins have been identified, as has a segment of the antigen (located near the alanine-rich amino-terminal portion) that can bind experimental pellicles39,40. The proline-rich central portion of antigen I/II might also have adhesin activity, on the basis of adherence-inhibition assays with fragments of recombinant antigen I/II (Ref. 41).
Secretory IgA specific for intact antigen I/II or for its salivary-protein-binding segment has been found to block adherence of S. mutans to saliva-coated hydroxyapatite20. Furthermore, active immunization42,43 with antigen I/II, as well as passive immunization15,17 with antigen-I/II-specific antibody, can protect rodents and non-human primates from experimental dental caries caused by S. mutans. Also, immunization of mice with synthetic peptides derived from the alanine-rich segment of antigen I/II has been shown to suppress colonization of teeth with S. mutan s44. Moreover, immunization with S. sobrinus surface protein antigen A (SpaA; which is similar to S. mutans antigen I/II) or with contructs of the adherence domain and structural region of S. mutans antigen I/II induces protective immunity against dental caries45,46. Protection afforded by immunization in each of these ways is thought to be mediated by interference in the initial colonization and by antibody-mediated agglutination and clearance of bacteria that express the adhesin.
GTFs. Early studies examined S. mutans with mutations in one of the genes encoding a GTF that synthesizes insoluble glucans, and these mutant bacteria were found to be relatively non-cariogenic compared with wild-type S. mutans47. Also, insertional inactivation of S. mutans gtfB and gtfC (which encode enzymes that synthesize insoluble glucans) or gtfD (which encodes a GTF that synthesizes soluble glucan) was shown to markedly reduce the incidence of dental caries48, indicating that both types of GTF have important roles in the pathogenesis of dental caries48.
The activity of GTFs is mediated through both catalytic and glucan-binding domains. The catalytic activity of GTFs is associated with at least two sites in the N-terminal third of the molecule49,50. The carboxy-terminal region of the GTF molecule contains a pattern of repeating sequences that are associated with glucan binding, and this pattern has been identified in all GTFs from mutans streptococci51,52. Immunization with synthetic peptides (in the form of multiple antigenic peptides, MAPs) that are derived from the sequence of catalytic or glucan-binding domains has been shown to elicit antibodies that can inhibit GTF activity53,54, and these MAP-based vaccines protect rats from experimentally induced dental caries55. Although the exact basis for experimental protection with such GTF-based vaccines is unknown, it seems probable that it involves functional inhibition of the catalytic and/or glucan-binding activity of GTF55. This suggestion is supported by the observation that co-immunization with GTF-derived peptides from the catalytic and the glucan-binding regions of GTF resulted in increased immune responses compared with immunization with either peptide alone, as well as in protection against dental caries56. More compelling is the finding that a diepitopic MAP containing peptide epitopes from both the catalytic and glucan-binding regions of GTF had a markedly higher immunogenicity than did a mixture of both peptides, giving rise to antibody that inhibited glucan formation by GTF and resulting in protection from dental caries57.
GBPs. Although several mutans streptococcal products with catalytic activities (such as GTFs and dextranases) can bind glucans, a separate group of proteins is synthesized that seems to function specifically as glucan receptors. These are known as GBPs, and they are present at the surface of mutans streptococci and participate in the formation of dental biofilms by functioning as receptors for glucans that have been synthesized by GTFs (Fig. 1b). Several S. mutans and S. sobrinus GBPs have been described58,59,60,61. All S. mutans strains that have been tested synthesize at least two GBPs, and one of these (GBP74) has substantial sequence homology to the glucan-binding domain of GTF62. An immunologically distinct S. mutans GBP (GbpB; also known as GBP59) has been shown to induce marked formation of specific IgA in the saliva of young children during natural infection63. Furthermore, immunization of rats with S. mutans GbpB induced protection against experimentally induced dental caries and inhibited colonization by S. mutans64. In addition, passive immunization with a chicken egg-yolk antibody specific for GbpB resulted in a substantial reduction in the incidence of experimentally induced dental caries65.
Clinical trials of vaccines
Active immunization. In the past decade, several small clinical trials of active immunization with mutans streptococcal antigens have been carried out. The goals of these studies have usually been to determine safety, to determine the concentration of mucosal antibody elicited, and to evaluate the possible effects on mutans streptococci that are indigenous or have been introduced.
The outcomes of clinical trials of vaccines with a single component — antigen I/II or intact GTF — are summarized in Table 1. In addition, the outcomes of preclinical studies resulting in protection against experimentally induced dental caries are also shown in Table 1, for both vaccines containing a single antigen (antigen I/II, intact GTF or GBP) and vaccines containing a combination of antigens (a GTF–glucan conjugate, GTF and antigen I/II administered as a mixture or a fusion protein, diepitopic constructs of peptides derived from the catalytic and glucan-binding regions of GTF, and diepitopic constructs of peptides derived from the catalytic region of GTF and a 20-amino-acid peptide derived from the N-terminal region of GbpB). Clinical trials have shown increased amounts of salivary secretory IgA specific for the mutans streptococcal antigen that was used for vaccination (either purified GTF13, or GTF and antigen I/II66,67,68,69,70) (Table 1). In the clinical trial of purified GTF, the vaccine group (members of which were immunized orally with GTF in capsules) showed substantially reduced reaccumulation of indigenous mutans streptococci for up to 42 days following tooth cleaning and vaccination13. Similar delayed reaccumulation was observed after topical application of GTF to the buccal mucosae, which contain the minor salivary glands and their ducts71. In addition, when antigen, either in soluble form or incorporated in liposomes, was administered intranasally or by topical application to the tonsils, the production of antigen-specific salivary IgA was induced70,72. However, the short-lived effects on mutans streptococci in the young adult populations that were studied indicates that immunization of this population would not affect the microbiota on a long-term basis (that is, for more than 42 days)13. Therefore, adults are not the appropriate target population for this vaccine.
With the clear requirement for a vaccine and with such strong vaccine-antigen candidates from animal studies, one might wonder why more trials of greater size have not been carried out. The usual argument against more extensive studies is that dental caries, a non-life-threatening disease, has a low financial priority. Although dental infections with mutans streptococci do not usually affect the survival of an individual, these infections are ubiquitous and have devastating effects, including pain, disfigurement and lost productivity, particularly in populations of disadvantaged children2. Nevertheless, there is a link between S. mutans and fatal infectious endocarditis73,74, with evidence to indicate that a vaccine might alleviate this condition75,76.
Passive immunization. Passive-immunization techniques, using transfer of milk antibody specific for whole mutans streptococci from mother to suckling infant, have been investigated in animal models and shown to be protective against dental caries7. Dietary antibody supplements78 (including chicken-egg-yolk antibody specific for S. mutans GTF79 or GbpB65) and topical application of monoclonal antibody80 have also been shown to interfere with the formation of dental caries. Studies of application of mouse monoclonal IgG specific for antigen I/II showed that recolonization with mutans streptococci was deferred for 2 years after a 9-day treatment with chlorhexidine prior to administration of antibody81 (Table 1). To extend this work, a transgenic tobacco plant producing human secretory IgA specific for antigen I/II was developed82. After treatment with chlorhexidine and passive application of the antibody, humans remained free of mutans streptococci for 4 months or longer. The mechanism by which colonization is inhibited has been suggested to involve the removal of sites in the biofilm that can be colonized by mutans streptococci, through allowing more extensive growth of competing microorganisms during the period of antibody application. However, recent preliminary investigations did not show any effects of this secretory antibody on the recurrence of mutans streptococci after chlorhexidine use83. Despite the uncertainties of the passive-immunization approach for prophylaxis of dental caries, the evidence provides strong confirmation of the effects of antibody on dental infection.
Target population for a vaccine
Although clinical trials have mostly been carried out in young adults (age 18–23)13,66,69,71, this is not the target population of choice, mainly because young adults are already infected with mutans streptococci (mainly S. mutans) and because the effects of antibody are transient13,14. On the basis of analysis of the natural history of oral colonization of young children with streptococci and of the ontogeny of the salivary immune response, the appropriate target population for a vaccine against dental caries is infants aged ∼12 months.
Colonization of the oral cavity with streptococci. Individuals are born with a sterile oral cavity but are rapidly colonized by maternal microbiota that can preferentially inhabit mucosal tissues and the saliva84 (Fig. 2a). The main components of the early microbiota of an infant are Streptococcus salivarius and Streptococcus mitis, both of which colonize an individual shortly after birth85,86,87. These soon constitute most of the streptococci in the oral cavity88, but the eruption of dentition at ∼6 months of age signals a considerable change in the characteristics and distribution of the colonizing microbiota6,89.
Teeth provide colonization sites for Streptococcus sanguis (also known as Streptococcus sanguinis) and mutans streptococci, as well as other tooth-inhabiting microorganisms. S. sanguis can be detected in most children by the end of the first year of life6,90,91 (Fig. 2a). Initial colonization of the mouth of a child with mutans streptococci usually occurs during a 'window of infectivity'92 between 18 and 36 months of age63. However, children can remain uninfected until the permanent dentition erupts63. Although an erupted primary dentition is required for colonization with mutans streptococci, it is not sufficient to ensure colonization. In addition to the infectious dose of mutans streptococci and the presence of teeth, other factors — such as the host immune response and the presence of dietary sucrose — have a role in the ultimate colonization of the child.
Ontogeny of the salivary mucosal immune response. The saliva of newborns is devoid of secretory IgA93. However, the concentration of secretory IgA rapidly increases and is close to that of adults by 1–2 years of age and certainly by 4–7 years of age63 (Fig. 2b). Therefore, colonization with oral bacteria occurs in a mucosal environment that is immunologically responsive to infectious challenge94. For example, by 3–5 weeks of age, salivary secretory IgA responses specific for microorganisms that colonize the oral cavity at this time (such as S. mitis and S. salivarius) can be detected63,88. By 12 months of age, both secretory IgA1 and secretory IgA2 specific for antigens of early colonizing streptococci are present91.
Serum IgG specific for S. mutans and S. mutans GTF is present in very small amounts between 1 and 3 years of age95 (Fig. 2c), which is when colonization with S. mutan s is taking place, and salivary IgA specific for S. mutans GTF can be found in less than 10% of children aged 1–3 years (Fig. 2b). So, although children between the ages of 1 and 3 years are immunologically competent with respect to mucosal immunity, they are often not infected with mutans streptococci at this time, and they do not produce antibody to mutans streptococcal GTF. Therefore, it might be possible to actively immunize children of this age with a mutans streptococcal antigen that is crucial in the molecular pathogenesis of dental caries (such as GTF). Such an approach could delay or prevent the effective colonization, establishment and accumulation of mutans streptococci. These procedures could be assisted by minimizing the maternally derived infectious mutans streptococcal challenge to which an infant is exposed before immunization, by administration of antibacterial agents, reduction of sucrose intake and physical removal of plaque31.
All of the data that we have discussed support the concept of immunization against dental caries. An ideal vaccine to alleviate dental caries would have the following features: the vaccine should consist of an antigen(s) that is involved in the molecular pathogenesis of dental caries; the vaccine should contain functionally important epitopes57,96; and the vaccine should be administered by a route that will reproducibly elicit mucosal antibody (intranasal and tonsillar routes being promising)67,70. Also, vaccination should occur when infants are immunocompetent with respect to salivary IgA production and before infection with mutans streptococci occurs. This is best accomplished when children are ∼12 months of age. One or more booster immunizations might be required. In our opinion, the ideal prototype vaccine would be a GTF–glucan conjugate96 (Table 1), which would be administered intranasally at ages 12 and 18 months. Such a conjugate vaccine has the advantages of being more effective at affording protection than GTF alone96 and of eliciting antibody specific for two components that are involved in the pathogenesis of dental caries, and it does so in the target population being addressed.
Conclusions: the moral imperative
Developing a vaccine against dental caries is feasible, and there is a moral obligation to implement this development because of the devastation this disease causes. Bioethicists have put forward a set of duties for health-science professionals: the duty of beneficence (to benefit society and, specifically, individuals); the duty of non-maleficence (while benefiting society, to avoid causing harm); the duty of respect for autonomy (to serve the best interest of others); and the duty of ensuring justice in individual and social contexts.
No vaccine is absolutely safe, and there is always a risk when providing medical intervention in a healthy individual to alleviate future disease. Those who are opposed to vaccination against dental caries argue that vaccination is not justifiable for a condition that is not life threatening. In addition, the pursuit of a vaccine against dental caries involves the commitment of resources, and the dental community is concerned that other approaches might be effective if adequately funded. The resolution of such concerns can only be achieved by clinical trials that are appropriately designed and carried out. But the role of clinical immunological science is to provide information for the public decision on the utility of a vaccine against dental caries, and the dental health of millions of children awaits these clinical trials. Despite these concerns, we think that biomedical scientists have an ethical obligation related to beneficence to pursue the development of a vaccine — that is, the obligation to benefit the oral health of society — and this is supported by the ethical obligation to promote (social) justice.
In A Theory of Justice97, Rawls asks one to envisage not knowing into what circumstances one will be born: that is, into a rich or poor family, being intelligent or dull, and so on. He argues that, given such a condition, people would design a world with some degree of risk aversion, with the following three conditions: each person would have an equal right to the most extensive system of liberties, with equal liberties for all; people with similar skills and abilities would have equal access to offices of society; and social and economic institutions would be arranged to provide maximal benefit to those who are the worst off. Given such a design of social justice, health-care systems would be committed to providing the most benefit to those who are the worst off, and dental caries disproportionately affects disadvantaged socio-economic groups, especially children in these groups2,4.
Furthermore, Daniels98 argues that a just society should provide basic health care to all but should redistribute health care more favourably to children. This conclusion is justified by the effect that health care has on the equality of opportunity for children (a fundamental requirement for justice). Children who are poor or belong to minority groups are the most vulnerable individuals in the United States, and they have the highest prevalence of dental caries and the least access to oral health care2. For justice to be achieved, these individuals need to receive maximum benefit from the health-care system so that they ultimately have an equal opportunity to thrive. Kopelman and Palumbo99 conclude that no matter which theoretical stance one takes, children should receive priority consideration in receiving health care. But our children do not even receive equal consideration, much less priority consideration.
A vaccine against dental caries offers great hope for rectifying this injustice, which deprives so many of the world's children of equal opportunity. Owing to economic, behavioural and cultural barriers, it is highly improbable that we will ever be able to reach the millions of susceptible children through the classic preventive armamentarium of water fluoridation, topical fluoride application, brushing and flossing, dental sealants and dietary-sucrose restriction. However, it is probable that most of these otherwise disenfranchised children could be reached with a vaccine against dental caries. Vaccines are particularly well suited for public-health applications in environments that do not lend themselves to regular health care. But, at present, initiatives for developing a vaccine against dental caries seem to be stymied, with major research resources directed to other agendas. Few, if any, issues in oral health research could be as compelling as the eradication or the reduction of dental caries.
Petersen, P. E. The World Oral Health Report 2003: Continuous Improvement of Oral Health in the 21st Century — The Approach of the WHO Global Oral Health Programme (World Health Organization, Geneva, 2003).
US Department of Health and Human Services. Oral Health in America: A Report of the Surgeon General (US Department of Health and Human Services, National Institute of Dental and Craniofacial Research, and National Institutes of Health, Rockville, 2000).
Hamada, S. & Slade, H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol. Rev. 44, 331–384 (1980).
Loesche, W. J. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50, 353–380 (1986).
Clark, J. K. On the bacterial factor in the etiology of dental caries. Br. J. Exp. Pathol. 5, 141–147 (1924).
Carlsson, P., Gandour, I. A., Olsson, B., Rickardsson, B. & Abbas, K. High prevalence of mutans streptococci in a population with extremely low prevalence of dental caries. Oral Microbiol. Immunol. 2, 121–124 (1987).
Hirasawa, M. & Takada, K. A new selective medium for Streptococcus mutans and the distribution of S. mutans and S. sobrinus and their serotypes in dental plaque. Caries Res. 37, 212–217 (2003).
Kohler, B., Bjarnason, S., Care, R., Mackevica, I. & Rence, I. Mutans streptococci and dental caries prevalence in a group of Latvian preschool children. Eur. J. Oral Sci. 103, 264–266 (1995).
Kristoffersson, K., Grondahl, H. G. & Bratthall, D. The more Streptococcus mutans, the more caries on approximal surfaces. J. Dent. Res. 64, 58–61 (1985).
Bratthall, D. in Risk Markers for Oral Diseases (ed. Johnson, N. W.) 287–312 (Cambridge Univ. Press, Cambridge, UK, 1991).
Taubman, M. A. in Comparative Immunology of the Oral Cavity (eds Mergenhagen, S. E. & Scherp, H. W.) 138–158 (US Government Printing Office, Washington DC, 1973).
Bowen, W. H. A vaccine against dental caries. A pilot experiment in monkeys (Macaca irus). Br. Dent. J. 126, 159–166 (1969).
Smith, D. J. & Taubman, M. A. Oral immunization of humans with Streptococcus sobrinus glucosyltransferase. Infect. Immun. 55, 2562–2569 (1987).
Gregory, R. L., Michalek, S. M., Filler, S. J., Mestecky, J. & McGhee, J. R. Prevention of Streptococcus mutans colonization by salivary IgA antibodies. J. Clin. Immunol. 5, 55–62 (1985).
Ma, J. K., Smith, R. & Lehner, T. Use of monoclonal antibodies in local passive immunization to prevent colonization of human teeth by Streptococcus mutans. Infect. Immun. 55, 1274–1278 (1987).
Staat, R. H., Langley, S. D. &, Doyle, R. J. Streptococcus mutans adherence: presumptive evidence for protein-mediated attachment followed by glucan-dependent cellular accumulation. Infect. Immun. 27, 675–681 (1980).
Russell, M. W. & Lehner, T. Characterisation of antigens extracted from cells and culture fluids of Streptococcus mutans serotype c. Arch. Oral Biol. 23, 7–15 (1978).
Lee, S. F., Progulske-Fox, A. & Bleiweis, A. S. Molecular cloning and expression of a Streptococcus mutans major surface protein antigen, P1 (I/II), in Escherichia coli. Infect. Immun. 56, 2114–2119 (1988).
Lamont, R. J., Demuth, D. R., Davis, C. A., Malamud, D. & Rosan, B. Salivary-agglutinin-mediated adherence of Streptococcus mutans to early plaque bacteria. Infect. Immun. 59, 3446–3450 (1991).
Hajishengallis, G., Nikolova, E. & Russell, M. W. Inhibition of Streptococcus mutans adherence to saliva-coated hydroxyapatite by human secretory immunoglobulin A (S-IgA) antibodies to cell surface protein antigen I/II: reversal by IgA1 protease cleavage. Infect. Immun. 60, 5057–5064 (1992).
Adjic, D. M. et al. Genome sequence of Streptococcus mutans UA 159, a cariogenic dental pathogen. Proc. Natl Acad. Sci. USA 99, 14434–14439 (2002).
Genco, R. J. & Taubman, M. A. Secretory γ-A antibodies induced by local immunization. Nature 221, 679–681 (1969).
Taubman, M. A. & Smith, D. J. Effects of local immunization with Streptococcus mutans on induction of salivary immunoglobulin A antibody experimental dental caries in rats. Infect. Immun. 9, 1079–1091 (1974).
McGhee, J. R. et al. Effective immunity to dental caries: protection of gnotobiotic rats by local immunization with Streptococcus mutans. J. Immunol. 16, 300–305 (1975).
Michalek, S. M., McGhee, J. R., Mestecky, J., Arnold, R. R. & Bozzo, L. Ingestion of S. mutans induces secretory IgA and caries immunity. Science 102, 1238–1240 (1976).
Lehner, T., Challacombe, S. J., Wilton, J. M. & Caldwell, J. Cellular and humoral immune responses in vaccination against dental caries in monkeys. Nature 264, 69–72 (1976).
Lehner, T., Russell, M. W., Caldwell, J. & Smith, R. Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infect. Immun. 34, 407–415 (1981).
Mestecky, J. et al. Selective induction of an immune response in human external secretions by ingestion of bacterial antigen. J. Clin. Invest. 61, 731–737 (1978).
Emmings, F. G., Evans, R. T. & Genco, R. J. Antibody response in the parotid fluid and serum of Irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans. Infect. Immun. 12, 281–292 (1975).
Evans, R. T., Emmings, F. G. & Genco, R. J. Prevention of Streptococcus mutans infection of tooth surfaces by salivary antibody in Irus monkeys (Macaca fascicularis). Infect. Immun. 12, 293–302 (1975).
Taubman, M. A. & Smith, D. J. in Contemporary Issues in Infectious Diseases Vol. 8 (eds Root, R. K., Warren, K. S., McCleod Griffiss, J. & Sande, M. A.) 99–112 (Churchill Livingstone, New York, 1989).
Michalek, S. M. & Childers, N. K. Development and outlook for a caries vaccine. Crit. Rev. Oral Biol. Med. 1, 37–54 (1990).
Taubman, M. A. & Smith, D. J. in Cariology for the Nineties (eds Bowen, W. H. & Tabak, L. A.) 441–457 (Univ. Rochester Press, Rochester, 1993).
Youngman, K. R., Lazarus, N. H. & Butcher, E. C. in Mucosal Immunology 3rd edn Vol. 1 (eds Mestecky, J. et al.) 667–680 (Elsevier, Burlington, 2005).
Culshaw, S. E., LaRosa, K., Eastcott, J. W., Smith, D. J. & Taubman, M. A. Novel immunogenic peptides selected for a caries vaccine by MHC-binding. J. Dent. Res. 83, 168 (2005).
Taubman, M. A., Smith, D. J., Ebersole, J. L. & Hillman, J. D. in Recent Advances in Mucosal Immunology (eds Strober, W., Hanson, L. A. & Sells, K. W.) 371–382 (Raven, New York, 1982).
Smith, D. J., van Houte, J., Kent, R. & Taubman, M. A. Effect of antibody in gingival crevicular fluid on early colonization of exposed root surfaces by mutans streptococci. Oral Microbiol. Immunol. 9, 65–69 (1994).
Okahashi, N., Sasakawa, C., Yoshikawa, M., Hamada, S. & Koga, T. Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans, implicated in dental caries. Mol. Microbiol. 3, 673–678 (1989).
Nakai, M., Okahashi, N., Ohta, H. & Koga, T. Saliva-binding region of Streptococcus mutans surface protein antigen. Infect. Immun. 61, 4344–4349 (1993).
Crowley, P. J., Brady, L. J., Piacentini, D. A. & Bleiweis, A. S. Identification of a salivary agglutinin-binding domain within cell surface adhesin P1 of Streptococcus mutans. Infect. Immun. 61, 1547–1552 (1993).
Lehner, T. et al. in Molecular Pathogenesis of Periodontal Disease (eds Genco, R. J., Hamada, S. Lehner, T., McGhee, J. R. & Mergenhagen, S.) 279–292 (American Society for Microbiology Press, Washington DC, 1994).
Lehner, T., Russell, M. W., Caldwell, J. & Smith, R. Immunization with purified protein antigens from Streptococcus mutans against dental caries in rhesus monkeys. Infect. Immun. 34, 407–415 (1981).
Katz, J. et al. Protective salivary immunoglobulin A responses against Streptococcus mutans infection after intranasal immunization with S. mutans antigen I/II coupled to the B subunit of cholera toxin. Infect. Immun. 61, 1964–1971 (1993).
Takahashi, I. et al. Immunogenicity and protective effect against oral colonization by Streptococcus mutans of synthetic peptides of a streptococcal surface protein antigen. J. Immunol. 146, 332–336 (1991).
Redman, T. K., Harmon, C. C., Lallone, R. L. & Michalek, S. M. Oral immunization with recombinant Salmonella typhimurium expressing surface protein antigen A of Streptococcus sobrinus: dose response and induction of protective humoral responses in rats. Infect. Immun. 63, 2004–2011 (1995).
Hajishengallis, G., Russell, M. W. & Michalek, S. M. Comparison of an adherence domain and a structural region of Streptococcus mutans antigen I/II in protective immunity against dental caries in rats after intranasal immunization. Infect. Immun. 66, 1740–1743 (1998).
Tanzer, J. M., Freedman, M. L., Fitzgerald, R. J. & Larson, R. H. Diminished virulence of glucan synthesis-defective mutants of Streptococcus mutans. Infect. Immun. 10, 197–203 (1974).
Yamashita, Y., Bowen, W. H., Burne, R. A. & Kuramitsu, H. K. Role of the Streptococcus mutans GFT genes in caries induction in the specific-pathogen-free rat model. Infect. Immun. 61, 3811–3817 (1993).
Mooser, G., Hefta, S. A., Paxton, R. J., Shively, J. E. & Lee, T. D. Isolation and sequence of an active-site peptide containing a catalytic aspartic acid from two Streptococcus sobrinus α-glucosyltransferases. J. Biol. Chem. 266, 8916–8922 (1991).
Funane, K., Shiraiwa, M., Hashimoto, K., Ichishima, E. & Kobayashi, M. An active-site peptide containing the second essential carboxyl group of dextransucrase from Leuconostoc mesenteroides by chemical modifications. Biochemistry 32, 13696–13702 (1993).
Ferretti, J. J., Gilpin, M. L. & Russell, R. R. Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28. J. Bacteriol. 169, 4271–4278 (1987).
Abo, H. et al. Peptide sequences for sucrose splitting and glucan binding within Streptococcus sobrinus glucosyltransferase (water-insoluble glucan synthetase). J. Bacteriol. 173, 989–996 (1991).
Smith, D. J. et al. Antigenicity and immunogenicity of a synthetic peptide derived from a glucan-binding domain of mutans streptococcal glucosyltransferase. Infect. Immun. 61, 2899–2905 (1993).
Smith, D. J. et al. Immunological characteristics of a synthetic peptide associated with a catalytic domain of mutans streptococcal glucosyltransferase. Infect. Immun. 62, 5470–5476 (1994).
Taubman, M. A., Holmberg, C. J. & Smith, D. J. Immunization of rats with synthetic peptide constructs from the glucan-binding or catalytic region of mutans streptococcal glucosyltransferase protects against dental caries. Infect. Immun. 63, 3088–3093 (1995).
Taubman, M. A., Smith, D. J., Holmberg, C. J. & Eastcott, J. W. Coimmunization with complementary glucosyltransferase peptides results in enhanced immunogenicity and protection against dental caries. Infect. Immun. 68, 2698–2703 (2000).
Taubman, M. A., Holmberg, C. J. & Smith, D. J. Diepitopic construct of functionally and epitopically complementary peptides enhances immunogenicity, reactivity with glucosyltransferase, and protection from dental caries. Infect. Immun. 69, 4210–4216 (2001).
Smith, D. J., Akita, H., King, W. F. & Taubman, M. A. Purification and antigenicity of a novel glucan-binding protein of Streptococcus mutans. Infect. Immun. 62, 2545–2552 (1994).
Russell, R. R. Glucan-binding proteins of Streptococcus mutans serotype c. J. Gen. Microbiol. 112, 197–201 (1979).
Landale, E. C. & McCabe, M. M. Characterization by affinity electrophoresis of an α-1,6-glucan-binding protein from Streptococcus sobrinus. Infect. Immun. 55, 3011–3016 (1987).
Wu-Yuan, C. D. & Gill, R. E. An 87-kilodalton glucan-binding protein of Streptococcus sobrinus B13. Infect. Immun. 60, 5291–5293 (1992).
Banas, J. A., Russell, R. R. & Ferretti, J. J. Sequence analysis of the gene for the glucan-binding protein of Streptococcus mutans Ingbritt. Infect. Immun. 58, 667–673 (1990).
Smith, D. J., King, W. F., Akita, H. & Taubman, M. A. Association of salivary immunoglobulin A antibody and initial mutans streptococcal infection. Oral Microbiol. Immunol. 13, 278–285 (1998).
Smith, D. J. & Taubman, M. A. Experimental immunization of rats with a Streptococcus mutans 59 kDa glucan-binding protein protects against dental caries. Infect. Immun. 64, 3069–3073 (1996).
Smith, D. J., King, W. F. & Godiska, R. Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries. Infect. Immun. 69, 3135–3142 (2001).
Childers, N. K., Tong, G. & Michalek, S. M. Nasal immunization of humans with dehydrated liposomes containing Streptococcus mutans antigen. Oral Microbiol. Immunol. 12, 329–335 (1997).
Childers, N. K. et al. A controlled clinical study of the effect of nasal immunization with a Streptococcus mutans antigen alone or incorporated into liposomes on induction of immune responses. Infect. Immun. 67, 618–623 (1999).
Childers, N. K., Zhang, S. S. & Michalek, S. M. Oral immunization of humans with dehydrated liposomes containing Streptococcus mutans glucosyltransferase induces salivary immunoglobulin A2 antibody responses. Oral Microbiol. Immunol. 9, 146–153 (1994).
Childers, N. K. et al. Effect of age on immunoglobulin A subclass distribution in human parotid saliva. Oral Microbiol. Immunol. 18, 298–301 (2003).
Childers, N. K. et al. Humans immunized with Streptococcus mutans antigens by mucosal routes. J. Dent. Res. 81, 48–52 (2002).
Smith, D. J. & Taubman, M. A. Effect of local deposition of antigen on salivary immune responses and reaccumulation of mutans streptococci. J. Clin. Immunol. 10, 273–281 (1990).
Fukuizumi, T., Inoue, H., Tsujisawa, T. & Uchiyama, C. Streptococcus sobrinus antigens that react to salivary antibodies induced by tonsillar application of formalin-killed S. sobrinus in rabbits. Infect. Immun. 68, 725–731 (2000).
Ullman, R. F., Miller, S. J., Stampfer, J. & Cunha, B. A. Streptococcus mutans endocarditis: report of three cases and review of the literature. Heart Lung 17, 209–212 (1988).
Mylonakis, F. & Calderwood, S. B. Infective endocarditis in adults. N. Engl. J. Med. 345, 1318–1330 (2001).
Munro, C. L. & Macrina, F. L. Sucrose-derived exopolysaccharides of Streptococcus mutans V403 contribute to infectivity in endocarditis. Mol. Microbiol. 8, 133–142 (1993).
Durack, D. T., Gilliland, B. C. & Petersdorf, R. F. Effect of immunization on susceptibility to experimental Streptococcus mutans and Streptococcus sanguis endocarditis. Infect. Immun. 22, 52–56 (1978).
Michalek, S. M. & McGhee, J. R. Effective immunity to dental caries: passive transfer to rats to antibodies to Streptococcus mutans elicits protection. Infect. Immun. 17, 644–650 (1977).
Michalek, S. M. et al. Protection of gnotobiotic rats against dental caries by passive immunization with bovine milk antibodies to Streptococcus mutans. Infect. Immun. 55, 2341–2347 (1987).
Hamada, S. et al. Oral passive immunization against dental caries in rats by use of hen egg yolk antibodies specific for cell-associated glucosyltransferase of Streptococcus mutans. Infect. Immun. 59, 4161–4167 (1991).
Lehner, T., Caldwell, J. & Smith, R. Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries. Infect. Immun. 50, 796–799 (1985).
Ma, J. K., Hunjan, M., Smith, R., Kelly, C. & Lehner, T. An investigation into the mechanism of protection by local passive immunization with monoclonal antibodies against Streptococcus mutans. Infect. Immun. 58, 3407–3414 (1990).
Ma, J. K. et al. Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nature Med. 4, 601–606 (1998).
Weintraub J. A. et al. Clinical trial of a plant-derived antibody on recolonization of mutans streptococci. Caries Res. 39, 241–250 (2005).
Gibbons, R. J. & van Houte, J. Selective bacterial adherence to oral epithelial surfaces and its role as an ecological determinant. Infect. Immun. 3, 567–573 (1971).
Carlsson, J., Grahnen, H. & Jonsson, G. Lactobacilli and streptococci in the mouth of children. Caries Res. 9, 333–339 (1975).
Carlsson, J., Grahnen, H., Jonsson, G. &, Wikner, S. Early establishment of Streptococcus salivarius in the mouth of infants. J. Dent. Res. 49, 415–418 (1970).
Pearce, C. et al. Identification of pioneer viridans streptococci in the oral cavity of human neonates. J. Med. Microbiol. 42, 67–72 (1995).
Smith, D. J., Anderson, J. M., King, W. F., van Houte, J. & Taubman, M. A. Oral streptococcal colonization of infants. Oral Microbiol. Immunol. 8, 1–4 (1993).
Kononen, E., Asikainen, S., Saarela, M., Karjalainen, J. & Jousimies-Somer, H. The oral Gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth. Oral Microbiol. Immunol. 9, 136–141 (1994).
Caufield, P. W., Dasanayake, A. P., Li, Y., Hsu, J. & Hardin, J. M. Natural history of Streptococcus sanguinis in the oral cavity of infants: evidence for a discrete window of infectivity. Infect. Immun. 68, 4283–4289 (2000).
Smith, D. J. & Taubman, M. A. Emergence of immune competence in saliva. Crit. Rev. Oral Biol. Med. 4, 335–341 (1993).
Caufield, P. W., Cutter, G. R. & Dasanayake, A. P. Initial acquisition of mutans streptococci by infants: evidence for a discrete window of infectivity. J. Dent. Res. 72, 37–45 (1993).
Gahnberg, L., Smith, D. J., Taubman, M. A. & Ebersole, J. L. Salivary-IgA antibody to glucosyltransferase of oral microbial origin in children. Arch. Oral Biol. 30, 551–556 (1985).
Smith, D. J. & Taubman, M. A. Ontogeny of immunity to oral microbiota in humans. Crit. Rev. Oral Biol. Med. 3, 109–133 (1992).
Luo, Z., Smith, D. J., Taubman, M. A. & King, W. F. Cross-sectional analysis of serum antibody to oral streptococcal antigens in children. J. Dent. Res. 67, 554–560 (1988).
Taubman, M. A., Smith, D. J., Holmberg, C. F. & Lees, A. GTF–S. sobrinus polysaccharide conjugates as potential caries vaccines. J. Dent. Res. 78, 453 (1999).
Rawls, J. A Theory of Justice (Harvard Univ. Press, Cambridge, Massachusetts, USA,1972).
Daniels, N. Just Health Care (Cambridge Univ. Press, Cambridge, UK, 1985).
Kopelman, L. M. & Palumbo, M. G. The U.S. health delivery system: inefficient and unfair to children. Am. J. Law Med. 23, 319–337 (1999).
Taubman, M. A. & Smith, D. J. Effects of local immunization with glucosyltransferase fractions from Streptococcus mutans on dental caries in rats and hamsters. J. Immunol. 118, 710–720 (1977).
Smith, D. J. et al. Remote glucosyltransferase-microparticle vaccine delivery induces protective immunity in the oral cavity. Oral Microbiol. Immunol. 18, 240–248 (2003).
Yu, H., Nakano, Y., Yamashita, Y., Ono, T. & Koga, T. Effects of antibodies against cell surface protein antigen Pac–glucosyltransferase fusion proteins on glucan synthesis and cell adhesion of Streptococcus mutans. Infect. Immun. 65, 2292–2298 (1997).
Zhang, P. et al. Enhance immunogenicity of a genetic chimeric protein consisting of two virulence antigens of Streptococcus mutans and protection against infection. Infect. Immun. 70, 6779–6787 (2002).
Smith, D. J., King, W. F., Rivero, J. & Taubman, M. A. Immunological and protective effects of diepitopic subunit dental caries vaccines. Infect. Immun. 73, 2797–2804 (2005).
Kaste, L. M. et al. Coronal caries in the primary and permanent dentition of children and adolescents 1–17 years of age: United States, 1988–1991. J. Dent. Res. 75, 631–641 (1996).
This work was supported by Public Health Service grants from the National Institute of Dental and Craniofacial Research (United States). The authors acknowledge the excellent assistance of L. Samit and M. LaMendola in compiling and preparing this manuscript.
The authors declare no competing financial interests.
A structure that is composed of populations or communities of microorganisms adhering to environmental surfaces (such as teeth).
An antimicrobial agent with a range of uses, including the treatment of gingivitis (gum inflammation), gum bleeding and periodontal infections (that is, infections of the gums, ligaments, bone and other tissues surrounding the teeth).
- Dental pellicle
A clear, thin tooth coating containing proteins, lipids and glycoproteins that are found in the saliva. Formation of a dental pellicle is the first step in formation of dental plaque.
- Diepitopic MAP
A multiple antigenic peptide (MAP) that contains two copies of each of two peptides.
The state of having no teeth.
- Egg-yolk antibody
An antibody this is extracted from chicken egg yolk after immunization of the chicken. In the chicken, the antibody passes from the serum to the egg yolk.
- Gingival crevicular fluid
The fluid that is present in the gingival crevice. It consists of a blood-derived transudate and/or exudate that contains antibodies from the serum and antibodies produced by plasma cells in the periodontal-pocket lamina.
- Multiple antigenic peptide
(MAP). An antigenic peptide construct that consists of a lysine backbone from which peptides are appended.
- Permanent dentition
The natural permanent teeth that replace the primary (deciduous) teeth.
- Stensen's duct
(Also known as the parotid duct). A conduit of the parotid gland in mammals. It carries saliva to the oral vestibule.
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Taubman, M., Nash, D. The scientific and public-health imperative for a vaccine against dental caries. Nat Rev Immunol 6, 555–563 (2006). https://doi.org/10.1038/nri1857
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