Persistent hepatitis C virus (HCV) infections — the main cause of liver disease worldwide — are a function of the ability of HCV to block its host's innate immune response. Expression of interferon (IFN), a key component of this defence, is induced by IFN regulatory factors (IRFs) after viral challenge. But what switches on the IRFs, and how does the virus interfere with this process to circumvent the immune response? Two studies published online in Science provide answers to these long-standing questions and highlight a promising target for drug development.
In the first study, Sharma and colleagues identified two IκB kinase (IKK)-related kinases that activate IRF-3 and IRF-7. When expressed in HEK293 cells, IKKε and TANK-binding kinase 1 (TBK1) activated the IRFs by phosphorylating the serine residue at position 396. Co-expression of IKKε or TBK1 with IRFs stimulated a variety of IFN promoters by up to 2,000-fold and inhibited virus replication in a de novo infection model, demonstrating the central function that IKKε and TBK1 have in activating host defences.
But these defences can be breached, and Foy et al. have shown that an HCV enzyme called NS3/4A does just that. NS3/4A is a protease complex that plays a crucial part in viral replication by catalysing the post-translational processing of non-structural HCV proteins. But it seems that NS3/4A has another function — blocking activation of IRF-3. Using osteosarcoma cells, Foy and colleagues showed that NS3/4A blocks the accumulation of activated IRF-3. In turn, induction of the IRF-3-responsive promoters of several IFN-stimulated genes is strongly inhibited in hepatoma cells. The authors suggest that NS3/4A inhibits IRF-3 activation by perturbing the enzymes that catalyse its phosphorylation — the newly identified IKK-related kinases, IKKε and TBK1.
As if it's not enough that NS3/4A-mediated inhibition of IRF-3 activation promotes the persistence of HCV infections by compromising the host's innate immune response, it probably also reduces the effectiveness of IFN-based therapies — the current standard treatment — as many IFN-responsive genes contain IRF-3 target sites within their promoters. So, antivirals that target NS3/4A might be doubly efficacious, restoring the capacity of the host to fight back, while weakening the virus by interfering with the protein processing that is essential to its replication.
References
ORIGINAL RESEARCH PAPERS
Foy, E. et al. Regulation of interferon regulatory factor-3 by the hepatitis C virus serine protease. Science 17 April 2003 (doi: 10.1126/science.1082604)
Sharma, S. et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 17 April 2003 (doi: 10.1126/science.1081315)
FURTHER READING
Tan, S. -T. et al. Hepatitis C therapeutics: current status and emerging therapies. Nature Rev. Drug Discov. 1, 867–881 (2002)
Related links
Rights and permissions
About this article
Cite this article
Farley, S. A double whammy for hep C. Nat Rev Drug Discov 2, 419 (2003). https://doi.org/10.1038/nrd1116
Issue Date:
DOI: https://doi.org/10.1038/nrd1116
This article is cited by
-
Modelling hepatitis C therapy—predicting effects of treatment
Nature Reviews Gastroenterology & Hepatology (2015)