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Spiral Tissue Microarrays as Next Evolutionary Step in the High-density Tissue Microarray Technology
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Spiral Tissue Microarrays as Next Evolutionary Step in the High-density Tissue Microarray Technology

  • Takeshi Uemura Dr.1,
  • Lucian Chirieac Dr.2,
  • Junya Fukuoka Prof.1,
  • Matthias Hofer Dr.2,
  • Takashi Hori1,
  • Tomonori Tanaka Dr.1,
  • Sayaka Tominaga1 &
  • …
  • Ryo Ohsawa1 

Nature Precedings (2010)Cite this article

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Abstract

Tissue microarray (TMA) is a well-established technique that connects basic research with clinical applications that allow the validation of many pathobiologic events from gene expression dysregulation to genomic aberrations. However, conventional TMAs have several limitations such as limited representation of tissue heterogeneity, destruction of donor tissue blocks due to coring and usage of particular specimens that have limited evaluable material (tissue from thin specimens or needle biopsies). We have developed a novel method, which we termed "Spiral TMA" that generates TMAs that allow for improved representation of the donor tissue while keeping the architectural details of the donor block intact. This technology is ideal for specimens with limited tissue without the need to punch holes into the original block and therefore preserving the tissue integrity. In this report, we describe the methodology of constructing Spiral TMA and demonstrate the validation of tumor representation and tissue heterogeneity by comparing Spiral TMA to conventional TMA using immunohistochemical staining to EGFR and CK7.

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  1. Toyama University Hospital, Department of Surgical Pathology https://www.nature.com/nature

    Takeshi Uemura Dr., Junya Fukuoka Prof., Takashi Hori, Tomonori Tanaka Dr., Sayaka Tominaga & Ryo Ohsawa

  2. Brigham and Women’s Hospital https://www.nature.com/nature

    Lucian Chirieac Dr. & Matthias Hofer Dr.

Authors
  1. Takeshi Uemura Dr.
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  2. Lucian Chirieac Dr.
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  3. Junya Fukuoka Prof.
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  4. Matthias Hofer Dr.
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  5. Takashi Hori
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  6. Tomonori Tanaka Dr.
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  7. Sayaka Tominaga
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  8. Ryo Ohsawa
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Corresponding author

Correspondence to Junya Fukuoka Prof..

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Uemura, T., Chirieac, L., Fukuoka, J. et al. Spiral Tissue Microarrays as Next Evolutionary Step in the High-density Tissue Microarray Technology. Nat Prec (2010). https://doi.org/10.1038/npre.2010.4831.1

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  • Received: 29 August 2010

  • Accepted: 30 August 2010

  • Published: 30 August 2010

  • DOI: https://doi.org/10.1038/npre.2010.4831.1

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Keywords

  • Microarray analysis
  • Heterogeneity
  • Cancer biomarker
  • pathology
  • biotechnology
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