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Expanded type III effector recognition by the ZAR1 NLR protein using ZED1-related kinases


Nucleotide-binding domain and leucine-rich repeat domain-containing (NLR) proteins are sentinels of plant immunity that monitor host proteins for perturbations induced by pathogenic effector proteins. Here we show that the Arabidopsis ZAR1 NLR protein requires the ZRK3 kinase to recognize the Pseudomonas syringae type III effector (T3E) HopF2a. These results support the hypothesis that ZAR1 associates with an expanded ZRK protein family to broaden its effector recognition spectrum.

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Figure 1: HopF2a induces ETI in Arabidopsis.
Figure 2: HopF2a recognition requires ZAR1 and ZRK3.


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We thank members of the Desveaux and Guttman laboratories for valuable input on the project. This work was supported by Ontario Graduate Scholarships and the Natural Sciences and Engineering Research Council of Canada (NSERC) postgraduate awards (D.S. and T.L.), NSERC Discovery Grants (D.D. and D.S.G.), a Canada Research Chair in Plant-Microbe Systems Biology (D.D.) or Comparative Genomics (D.S.G.) and the Centre for the Analysis of Genome Evolution and Function (D.D. and D.S.G.). T-DNA lines were obtained from the ABRC.

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D.S., N.K., T.L., A.M. and D.D. designed experiments. D.S., N.K., T.L. and A.M. conducted experiments. D.S., N.K., T.L., A.M., D.S.G. and D.D. analysed data. D.S. and D.D. wrote the manuscript.

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Correspondence to Darrell Desveaux.

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The authors declare no competing financial interests.

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Supplementary Methods, Supplementary References, Supplementary Figure Legends 1–4 and Supplementary Figures 1–4. (PDF 10830 kb)

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Seto, D., Koulena, N., Lo, T. et al. Expanded type III effector recognition by the ZAR1 NLR protein using ZED1-related kinases. Nature Plants 3, 17027 (2017).

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