Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)–Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA polymerase II promoter expression system. LbCpf1 generated biallelic mutations at nearly 100% efficiency at four independent sites in rice T0 transgenic plants. Moreover, we repurposed AsCpf1 and LbCpf1 for efficient transcriptional repression in Arabidopsis, and demonstrated a more than tenfold reduction in miR159b transcription. Our data suggest promising applications of CRISPR–Cpf1 for editing plant genomes and modulating the plant transcriptome.
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Acknowledgements
This work was supported by grants including the National Science Foundation of China (31330017, 31271420 and 31371682) and the National Transgenic Major Project (2014ZX0801003B-002) to X.Z. and Y.Z., a Collaborative Funding Grant from North Carolina Biotechnology Center and Syngenta Biotechnology (2016-CFG-8003) and a start-up fund from University of Maryland-College Park to Y.Q., and National Science Foundation (MCB 0209818, DBI 0923827 and 105-1339209) to D.F.V.
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Contributions
Y.Q., Y.Z. and D.F.V. designed the experiments. Y.Q., L.G.L. and A.A.M. generated all the constructs. X.T. and Y.Z. performed the transient assays in protoplasts and prepared samples for deep sequencing. T.Z., Y.Z. and X.T. analysed the deep sequencing data. X.T., X.Z., Z.Z., Y.C., Q.R. and Q.L. generated stable transgenic rice and analysed the plants. L.G.L. and E.R.K. produced Arabidopsis transcriptional repression data. Y.Q., Y.Z. and D.F.V. wrote the paper with input from other authors. All authors read and approved the final manuscript.
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Supplementary Information
Supplementary Methods, Supplementary References, Supplementary Figures 1–10, Supplementary Table 1 (Oligos and gBlocks used in this study). (PDF 3235 kb)
Supplementary Table 2
Assembled T-DNA vectors used in this study. (XLSX 11 kb)
Supplementary Table 3
Sample information for high-throughput sequencing. (XLS 14 kb)
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Tang, X., Lowder, L., Zhang, T. et al. A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants. Nature Plants 3, 17018 (2017). https://doi.org/10.1038/nplants.2017.18
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DOI: https://doi.org/10.1038/nplants.2017.18
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