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The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein

An Author Correction to this article was published on 23 October 2017

Abstract

Plants and animals recognize microbial invaders by detecting microbe-associated molecular patterns (MAMPs) by cell surface receptors. Many plant species of the Solanaceae family detect the highly conserved nucleic acid binding motif RNP-1 of bacterial cold-shock proteins (CSPs), represented by the peptide csp22, as a MAMP. Here, we exploited the natural variation in csp22 perception observed between cultivated tomato (Solanum lycopersicum) and Solanum pennellii to map and identify the leucine-rich repeat (LRR) receptor kinase CORE (cold shock protein receptor) of tomato as the specific, high-affinity receptor site for csp22. Corroborating its function as a genuine receptor, heterologous expression of CORE in Arabidopsis thaliana conferred full sensitivity to csp22 and, importantly, it also rendered these plants more resistant to infection by the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Our study also confirms the biotechnological potential of enhancing plant immunity by interspecies transfer of highly effective pattern-recognition receptors such as CORE to different plant families.

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Figure 1: Csp22-dependent induction of ethylene biosynthesis in tomato (S. lycopersicum), S. pennellii and a collection of recombinant inbred lines.
Figure 2: Csp22-dependent induction of MAMP responses in non-transformed N. benthamiana and in N. benthamiana expressing receptor candidate genes from tomato (S. lycopersicum).
Figure 3: CORE forms a complex with SERK3 in response to treatment with csp22 or csp15.
Figure 4: CORE binds csp22 and csp15 with high affinity and high specificity.
Figure 5: The receptor kinase CORE is sufficient to confer responsiveness to csp22 when expressed in cells of A. thaliana.
Figure 6: The receptor kinase CORE expressed in A. thaliana is fully functional and contributes to resistance against the bacterial pathogen Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000).

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Acknowledgements

The authors are grateful to I. Bock (Univ. Tübingen) for technical assistance, to H. Kalbacher (Univ. Tübingen) for helping with the synthesis of acri-csp22, to S. Robatzek (TSL, Norwich) for supplying SlSERK3a-myc and to J. Fliegmann (Univ. Tübingen) and D. Chinchilla (Univ. Basel) for critical reading of the manuscript. This work was supported by BMBF-KBBE project 031A328 and by Deutsche Forschungsgemeinschaft through CRC 1101.

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L.W., M.A., E.E., U.F., D.K. and G.F. performed experiments and analysed data; L.W., M.A. and G.F. designed experiments; L.W., M.A. and G.F. wrote the manuscript.

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Correspondence to Georg Felix.

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Supplementary Figures 1–6, Supplementary Table 1. (PDF 542 kb)

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Wang, L., Albert, M., Einig, E. et al. The pattern-recognition receptor CORE of Solanaceae detects bacterial cold-shock protein. Nature Plants 2, 16185 (2016). https://doi.org/10.1038/nplants.2016.185

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