The mechanism for T-DNA integration, a critical step of Agrobacterium-mediated transgenesis, remains poorly understood. Now, a study based on mutant analysis shows that Pol θ controls T-DNA integration and generates error-prone footprints at integration sites.
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Levy, A. T-DNA integration: Pol θ controls T-DNA integration. Nature Plants 2, 16170 (2016). https://doi.org/10.1038/nplants.2016.170
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DOI: https://doi.org/10.1038/nplants.2016.170