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Light-guiding hydrogels for cell-based sensing and optogenetic synthesis in vivo

Abstract

Polymer hydrogels are widely used as cell scaffolds for biomedical applications. Although the biochemical and biophysical properties of hydrogels have been investigated extensively, little attention has been paid to their potential photonic functionalities. Here, we report cell-integrated polyethylene glycol-based hydrogels for in vivo optical-sensing and therapy applications. Hydrogel patches containing cells were implanted in awake, freely moving mice for several days and shown to offer long-term transparency, biocompatibility, cell viability and light-guiding properties (loss of <1 dB cm−1). Using optogenetic, glucagon-like peptide-1 secreting cells, we conducted light-controlled therapy using the hydrogel in a mouse model with diabetes and obtained improved glucose homeostasis. Furthermore, real-time optical readout of encapsulated heat-shock-protein-coupled fluorescent reporter cells made it possible to measure the nanotoxicity of cadmium-based bare and shelled quantum dots (CdTe; CdSe/ZnS) in vivo.

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Figure 1: Schematic of a light-guiding hydrogel encapsulating cells for in vivo sensing and therapy.
Figure 2: Characteristics of hydrogels.
Figure 3: Light-guiding properties of fibre-optic hydrogels.
Figure 4: Hydrogel implants in vivo.
Figure 5: Cell-based sensing of nanocytotoxicity of quantum dots.
Figure 6: Optogenetic therapy in a mouse model of diabetes.

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Acknowledgements

The authors thank M. Fussenegger and H. Ye (ETH) for providing plasmids for optogenetic experiments. This work was funded by the US National Institutes of Health (R21 EB013761), the US National Science Foundation (ECS-1101947), the US Department of Defense (FA9550-10-1-0537), the IT Consilience Creative Program of MKE and NIPA (C1515-1121-0003) and the Bio & Medical Technology Development Program and the World Class University Program of the Korean National Research Foundation (2012M3A9C6049791 and R31-2008-000-10071-0). S.N. acknowledges financial support from the Bullock–Wellman Fellowship.

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M.C. and S.H.Y. designed the experiments. M.C. performed the experiments. J.W.C., S.K. and S.N. provided materials. M.C., S.K., S.K.H. and S.H.Y. analysed the data. M.C. and S.H.Y. wrote the manuscript, with input from all authors.

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Correspondence to Seok Hyun Yun.

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The authors declare no competing financial interests.

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Choi, M., Choi, J., Kim, S. et al. Light-guiding hydrogels for cell-based sensing and optogenetic synthesis in vivo. Nature Photon 7, 987–994 (2013). https://doi.org/10.1038/nphoton.2013.278

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