Acoustically induced inelastic light scattering, first reported in 1922 by Brillouin1, allows non-contact, direct readout of the viscoelastic properties of a material and has widely been investigated for material characterization2, structural monitoring3 and environmental sensing4. Extending the Brillouin technique from point sampling spectroscopy to imaging modality5 would open up new possibilities for mechanical imaging, but has been challenging because rapid spectrum acquisition is required. Here, we demonstrate a confocal Brillouin microscope based on a fully parallel spectrometer—a virtually imaged phased array—that improves the detection efficiency by nearly 100-fold over previous approaches. Using the system, we show the first cross-sectional Brillouin imaging based on elastic properties as the contrast mechanism and monitor fast dynamic changes in elastic modulus during polymer crosslinking. Furthermore, we report the first in situ biomechanical measurement of the crystalline lens in a mouse eye. These results suggest multiple applications of Brillouin microscopy in biomedical and biomaterial science.
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Brillouin, L. Diffusion de la lumiere et des rayonnes X par un corps transparent homogene; influence del'agitation thermique. Ann. Phys. (French) 17, 88–122 (1922).
Dil, J. G. Brillouin-scattering in condensed matter. Rep. Prog. Phys. 45, 285–334 (1982).
Culshaw, B., Michie, C., Gardiner, P. & McGown, A. Smart structures and applications in civil engineering. Proc. IEEE 84, 78–86 (1996).
Eloranta, E. W. High spectral resolution lidar. in Lidar: Range-Resolved Optical Remote Sensing of the Atmosphere 143–164 (ed. Weitkamp, C.) (Springer-Verlag, New York, 2005).
Koski, K. J. & Yarger, J. L. Brillouin imaging. Appl. Phys. Lett. 87, 061903 (2005).
Fung, Y. C. Biomechanics: Mechanical Properties of Living Tissues (Springer-Verlag, New York, 1993).
Comoglio, P. M. & Trusolino, L. Cancer: the matrix is now in control. Nature Med. 11, 1156–1159 (2005).
Langer, R. & Tirrell, D. A. Designing materials for biology and medicine. Nature 428, 487–492 (2004).
Claessens, M. M. A. E., Tharmann, R., Kroy, K. & Bausch, A. R. Microstructure and viscoelasticity of confined serniflexible polymer networks. Nature Phys. 2, 186–189 (2006).
Bao, G. & Suresh, S. Cell and molecular mechanics of biological materials. Nature Mater. 2, 715–725 (2003).
Ophir, J., Cespedes, I., Ponnekanti, H., Yazdi, Y. & Li, X. Elastography—a quantitative method for imaging the elasticity of biological tissues. Ultrason. Imaging 13, 111–134 (1991).
Greenleaf, J. F., Fatemi, M. & Insana, M. Selected methods for imaging elastic properties of biological tissues. Ann. Rev. Biomed. Eng. 5, 57–78 (2003).
Harley, R., James, D., Miller, A. & White, J. W. Phonons and elastic-moduli of collagen and muscle. Nature 267, 285–287 (1977).
Randall, J. & Vaughan, J. M. Brillouin scattering in systems of biological significance. Phil. Trans. R. Soc. Lond. A 293, 341–348 (1979).
Lees, S., Tao, N. J. & Lindsay, S. M. Studies of compact hard tissues and collagen by means of Brillouin light-scattering. Connect. Tissue Res. 24, 187–205 (1990).
Vaughan, J. M. & Randall, J. T. Brillouin scattering, density and elastic properties of the lens and cornea of the eye. Nature 284, 489–491 (1980).
Randall, J. & Vaughan, J. M. The measurement and interpretation of Brillouin scattering in the lens of the eye. Proc. R. Soc. Lond. B 214, 449–470 (1982).
Lindsay, S. M., Anderson, M. W. & Sandercock, J. R. Construction and alignment of a high-performance multipass vernier tandem Fabry–Perot interferometer. Rev. Sci. Instrum. 52, 1478–1486 (1981).
Benassi, P., Eramo, R., Giugni, A., Nardone, M. & Sampoli, M. A spectrometer for high-resolution and high-contrast Brillouin spectroscopy in the ultraviolet. Rev. Sci. Instrum. 76, 013904 (2005).
Tanaka, H. & Sonehara, T. New method of superheterodyne light beating spectroscopy for Brillouin scattering using frequency-tunable lasers. Phys. Rev. Lett. 74, 1609–1612 (1995).
Itoh, S. Very rapid nonscanning Brillouin spectroscopy using fixed etalons and multichannel detectors. Jpn J. Appl. Phys. 37, 3134–3135 (1998).
Shirasaki, M. Large angular dispersion by a virtually imaged phased array and its application to a wavelength demultiplexer. Opt. Lett. 21, 366–368 (1996).
Diddams, S. A., Hollberg, L. & Mbele, V. Molecular fingerprinting with the resolved modes of a femtosecond laser frequency comb. Nature 445, 627–630 (2007).
Wang, T. D., Mandella, M. J., Contag, C. H. & Kino, G. S. Dual-axis confocal microscope for high-resolution in vivo imaging. Opt. Lett. 28, 414–416 (2003).
Danielmeyer, H. G. Aperture corrections for sound-absorption measurements with light scattering. J. Acoust. Soc. Am. 47, 151–154 (1970).
Faris, G. W., Jusinski, L. E. & Hickman, A. P. High-resolution stimulated Brillouin gain spectroscopy in glasses and crystals. J. Opt. Soc. Am. B 10, 587–599 (1993).
Ahsan, T., Harwood, F., McGowan, K. B., Amiel, D. & Sah, R. L. Kinetics of collagen crosslinking in adult bovine articular cartilage. Osteoarth. Cart. 13, 709–715 (2005).
Zumbusch, A., Holtom, G. R. & Xie, X. S. Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering. Phys. Rev. Lett. 82, 4142–4145 (1999).
Heys, K. R., Cram, S. L. & Truscott, R. J. W. Massive increase in the stiffness of the human lens nucleus with age: the basis for presbyopia? Mol. Vis. 10, 956–963 (2004).
Ethier, C. R., Johnson, M. & Ruberti, J. Ocular biomechanics and biotransport. Ann. Rev. Biomed. Eng. 6, 249–273 (2004).
This work was supported by the US Department of Defense (FA9550-04-1-0079) and the Center for Integration of Medicine and Innovative Technologies (CIMIT). We thank P. Kim for preparing the eye sample, C.P. Lin for lending us the CCD camera, and I.E. Kochevar and R.W. Redmond for helpful comments.
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Scarcelli, G., Yun, S. Confocal Brillouin microscopy for three-dimensional mechanical imaging. Nature Photon 2, 39–43 (2008) doi:10.1038/nphoton.2007.250
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