Article

Biphasic growth dynamics control cell division in Caulobacter crescentus

  • Nature Microbiology 2, Article number: 17116 (2017)
  • doi:10.1038/nmicrobiol.2017.116
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Abstract

Cell size is specific to each species and impacts cell function. Various phenomenological models for cell size regulation have been proposed, but recent work in bacteria has suggested an ‘adder’ model, in which a cell increments its size by a constant amount between each division. However, the coupling between cell size, shape and constriction remains poorly understood. Here, we investigate size control and the cell cycle dependence of bacterial growth using multigenerational cell growth and shape data for single Caulobacter crescentus cells. Our analysis reveals a biphasic mode of growth: a relative timer phase before constriction where cell growth is correlated to its initial size, followed by a pure adder phase during constriction. Cell wall labelling measurements reinforce this biphasic model, in which a crossover from uniform lateral growth to localized septal growth is observed. We present a mathematical model that quantitatively explains this biphasic ‘mixer’ model for cell size control.

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Acknowledgements

The authors thank C. Wright and S. Iyer-Biswas for measurements and shape analysis of C. crescentus single-cell data1,2. The authors thank S. Crosson and A. Fiebig for contributing reagents, materials and discussions. The authors acknowledge funding from the National Science Foundation Physics of Living Systems (NSF PHY-1305542), the National Science Foundation Materials Research Science and Engineering Center (MRSEC) at the University of Chicago (NSF DMR-1420709), the W. M. Keck Foundation and the Graduate Program in Biophysical Sciences at the University of Chicago (T32 EB009412/EB/NIBIB NIH HHS/United States). S.B. acknowledges support from the University College London for completion of part of this work.

Author information

Affiliations

  1. James Franck Institute, The University of Chicago, Chicago, Illinois 60637, USA

    • Shiladitya Banerjee
    • , Klevin Lo
    • , Aaron R. Dinner
    •  & Norbert F. Scherer
  2. Department of Physics and Astronomy, University College London, London WC1E 6BT, UK

    • Shiladitya Banerjee
  3. Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UK

    • Shiladitya Banerjee
  4. Institute for Biophysical Dynamics, The University of Chicago, Chicago, llinois 60637, USA

    • Klevin Lo
    • , Matthew K. Daddysman
    • , Alan Selewa
    • , Aaron R. Dinner
    •  & Norbert F. Scherer
  5. Biophysical Sciences Graduate Program, The University of Chicago, Chicago, Illinois 60637, USA

    • Alan Selewa
  6. Department of Chemistry, The University of Chicago, Chicago, Illinois 60637, USA

    • Thomas Kuntz
    • , Aaron R. Dinner
    •  & Norbert F. Scherer

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Contributions

S.B., K.L., A.R.D. and N.F.S. designed the research. S.B., K.L., A.S., M.K.D. and T.K. performed the research. S.B., A.R.D. and N.F.S. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Aaron R. Dinner or Norbert F. Scherer.

Supplementary information

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    Supplementary Information

    Supplementary Methods, Supplementary Notes 1–6, Supplementary Discussion, Supplementary References, Supplementary Figures 1–13.