Brief Communication

Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy

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Abstract

Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.

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Author information

Author notes

    • Yoshifumi Wakisaka
    •  & Yuta Suzuki

    These authors contributed equally to this work.

Affiliations

  1. Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan

    • Yoshifumi Wakisaka
    •  & Keisuke Goda
  2. Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo 113-8656, Japan

    • Yuta Suzuki
    •  & Yasuyuki Ozeki
  3. Research & Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan

    • Osamu Iwata
    • , Ayaka Nakashima
    •  & Kengo Suzuki
  4. Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan

    • Takuro Ito
  5. Graduate School of Media and Governance, Keio University, Fujisawa, Kanagawa 252-8520, Japan

    • Takuro Ito
  6. Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan

    • Misa Hirose
    • , Ryota Domon
    • , Mai Sugawara
    •  & Norimichi Tsumura
  7. Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan

    • Hiroshi Watarai
  8. Department of Electrical and Electronics Engineering, Chiba University, Chiba 263-8522, Japan

    • Tomoyoshi Shimobaba
  9. Department of Electrical Engineering, University of California, Los Angeles, California 90095, USA

    • Keisuke Goda
  10. Japan Science and Technology Agency, Tokyo 102-8666, Japan

    • Keisuke Goda

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Contributions

Y.W. and Y.S. performed the experiments. O.I., A.N. and T.I. prepared the samples. Y.W., Y.S., M.H., R.D., M.S., N.T., T.S. and H.W. performed the data analysis. Y.W., Y.S., K.G. and Y.O. wrote the manuscript. K.G. conceived the concept. K.S., K.G. and Y.O. supervised the work.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Keisuke Goda or Yasuyuki Ozeki.

Supplementary information

PDF files

  1. 1.

    Supplementary information

    Supplementary Discussion, Supplementary References, Supplementary Figures 1–8, Legends for Supplementary Videos 1 and 2.

Videos

  1. 1.

    Supplementary Video 1

    Comparison in SRS imaging of motile E. gracilis in motion between frame rates of 27 fps (top) and 6.75 fps (bottom).

  2. 2.

    Supplementary Video 1

    3D metabolite imaging of E. gracilis.