Abstract
Protein-protein interactions are fundamental to virtually every aspect of cellular functions. Blue, green and yellow bimolecular fluorescence complementation (BiFC) systems based on GFP and its variants allow the investigation of protein-protein interactions in vivo. We have developed the first red BiFC system based on an improved monomeric red fluorescent protein (mRFP1-Q66T), expanding the range of possible applications for BiFC.
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Acknowledgements
We thank G. Coupland and M. Hülskamp for support, and M. Mutondo for critically reading the manuscript. The work was supported by grants from the Max Planck Society.
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Supplementary information
Supplementary Fig. 1
Analysis of negative controls (PDF 1891 kb)
Supplementary Fig. 2
Analysis of homeodomain protein interactions (PDF 70 kb)
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Jach, G., Pesch, M., Richter, K. et al. An improved mRFP1 adds red to bimolecular fluorescence complementation. Nat Methods 3, 597–600 (2006). https://doi.org/10.1038/nmeth901
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DOI: https://doi.org/10.1038/nmeth901
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