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BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions

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Figure 1
Figure 2: Phase contrast micrograph (viewed at 400×) of emulsions deposited in the well of a 48-well tissue culture plate.
Figure 3: Analysis of bead populations with flow cytometry.

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Correspondence to Bert Vogelstein.

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Under a licensing agreement between EXACT Sciences and The Jonhs Hopkins University, K.W.K. and B.V. are entitled to a share of royalties received by the university on sales of products related to digital PCR. Under a licensing agreement between Agencourt Biosciences Corporation and The Jonhs Hopkins University, D.D., K.W.K. and B.V. are entitled to a share of royalties received by the university on sales of products related to the use of BEAMing for preparing templates for DNA sequencing. The terms of these arrangements are being managed by The Jonhs Hopkins University in accordance with its conflict of interest policies.

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Diehl, F., Li, M., He, Y. et al. BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions. Nat Methods 3, 551–559 (2006). https://doi.org/10.1038/nmeth898

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