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High-throughput RNAi screening by time-lapse imaging of live human cells

Nature Methods volume 3, pages 385390 (2006) | Download Citation

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Abstract

RNA interference (RNAi) is a powerful tool to study gene function in cultured cells. Transfected cell microarrays in principle allow high-throughput phenotypic analysis after gene knockdown by microscopy. But bottlenecks in imaging and data analysis have limited such high-content screens to endpoint assays in fixed cells and determination of global parameters such as viability. Here we have overcome these limitations and developed an automated platform for high-content RNAi screening by time-lapse fluorescence microscopy of live HeLa cells expressing histone-GFP to report on chromosome segregation and structure. We automated all steps, including printing transfection-ready small interfering RNA (siRNA) microarrays, fluorescence imaging and computational phenotyping of digital images, in a high-throughput workflow. We validated this method in a pilot screen assaying cell division and delivered a sensitive, time-resolved phenoprint for each of the 49 endogenous genes we suppressed. This modular platform is scalable and makes the power of time-lapse microscopy available for genome-wide RNAi screens.

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Acknowledgements

We thank S. Narumiya (Kyoto University, Kyoto) and T. Hirota (Institute of Molecular Pathology; IMP; Vienna) for HeLa 'Kyoto' cells; W. Huber (European Bioinformatics Institute; EBI; Hinxton) for advice on statistical analysis of kinetic data; O. Gruss (Zentrum für Molekulare Biologie Heidelberg; ZNBH; Heidelberg) for TPX2 antibody; J.-M. Peters (IMP; Vienna) for RPE cells; I. Hoffmann (Deutsches Krebsforschungszentrum; DKFZ; Heidelberg) for U2OS cells; H. Runz (Univ. Heidelberg) for primary human fibroblasts; Chroma Inc. for providing customized emission filter sets free of charge; EMBL's IT Services group (B. Kindler, M. Hemberger, R. Lück) for support; Olympus Biosystems, Hamilton and Bio-Rad for continuous support; Cenix BioScience GmbH for siRNA design and for providing the A549 cells; and Ambion Europe, Ltd. for providing siRNAs for validation. This project was funded by grants to J.E. within the MitoCheck consortium by the European Commission (FP6-503464) as well as in part by the Federal Ministry of Education and Research (BMBF) in the framework of the National Genome Research Network (NGFN) (NGFN-2 SMP-RNAi, FKZ01GR0403 to J.E. and NGFN-2 SMP-Cell FKZ01GR0423, NGFN-1 FKZ01GR0101, FKZ01KW0013 to R.P.).

Author information

Author notes

    • Beate Neumann
    • , Michael Held
    • , Urban Liebel
    •  & Holger Erfle

    These authors contributed equally to this work.

Affiliations

  1. MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

    • Beate Neumann
    • , Michael Held
    • , Urban Liebel
    • , Holger Erfle
    •  & Phill Rogers
  2. Cell Biology/Biophysics, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

    • Rainer Pepperkok
    •  & Jan Ellenberg
  3. Gene Expression Programmes, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

    • Jan Ellenberg

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Jan Ellenberg.

Supplementary information

PDF files

  1. 1.

    Supplementary Fig. 1

    siRNA knock-down efficiency.

  2. 2.

    Supplementary Fig. 2

    Examples of detected RNAi phenotypes.

  3. 3.

    Supplementary Table 1

    Summary of siRNA sequences

  4. 4.

    Supplementary Table 2

    Summary of RT-PCR Primer

  5. 5.

    Supplementary Methods

  6. 6.

    Supplementary Protocol

Videos

  1. 1.

    Supplementary Video 1

    siRNA SCRAMBLED

  2. 2.

    Supplementary Video 2

    siRNA INCENP - Segregation problem leading to multinucleated cells

  3. 3.

    Supplementary Video 3

    siRNA SYNE2 - Metaphase alignment problem followed by segregation followed by apoptosis

  4. 4.

    Supplementary Video 4

    siRNA PLK1 - Prometaphase arrest followed by apoptosis

  5. 5.

    Supplementary Video 5

    siRNA CDC16 - Metaphase alignment problems followed by apoptosis

  6. 6.

    Supplementary Video 6

    siRNA NUP107 - Metaphase alignment problems followed by apoptosis

  7. 7.

    Supplementary Video 7

    siRNA NUMA1 - Apoptosis

About this article

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DOI

https://doi.org/10.1038/nmeth876

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