Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.
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We thank P. Kapahi for critical comments and valuable suggestions. This work was supported by a grant from the US National Institutes of Health (AG20438).
The authors declare no competing financial interests.
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Garcia, A., Derventzi, A., Busuttil, R. et al. A model system for analyzing somatic mutations in Drosophila melanogaster. Nat Methods 4, 401–403 (2007) doi:10.1038/nmeth1027
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