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Mass spectrometry in high-throughput proteomics: ready for the big time

Nature Methods volume 7, pages 681–685 (2010)Cite this article

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Mass spectrometry has evolved and matured to a level where it is able to assess the complexity of the human proteome. We discuss some of the expected challenges ahead and promising strategies for success.

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Author information

Authors and Affiliations

  1. The Research Institute of the McGill University Health Centre, McGill University, Montreal, Quebec, Canada

    Tommy Nilsson & John J M Bergeron

  2. Department of Medicine, McGill University, Montreal, Quebec, Canada

    Tommy Nilsson & John J M Bergeron

  3. Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany

    Matthias Mann

  4. Institute of Molecular Systems Biology, Swiss Federal Institute of Technology (ETH) Zurich, Zurich, Switzerland

    Ruedi Aebersold

  5. Faculty of Science, University of Zurich, Zurich, Switzerland

    Ruedi Aebersold

  6. Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA

    John R Yates III

  7. Swiss Institute of Bioinformatics, Geneva, Switzerland

    Amos Bairoch

  8. University of Geneva, Geneva, Switzerland

    Amos Bairoch

Authors
  1. Tommy Nilsson
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  2. Matthias Mann
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  3. Ruedi Aebersold
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  4. John R Yates III
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  5. Amos Bairoch
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  6. John J M Bergeron
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Corresponding authors

Correspondence to Tommy Nilsson or John J M Bergeron.

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The authors declare no competing financial interests.

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Nilsson, T., Mann, M., Aebersold, R. et al. Mass spectrometry in high-throughput proteomics: ready for the big time. Nat Methods 7, 681–685 (2010). https://doi.org/10.1038/nmeth0910-681

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