Targeted genomic insertion will improve the qualitative and quantitative functional comparison of similar transgenes and provide suitable integration points for transgenes of applied interest.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii
BMC Genetics Open Access 18 December 2020
-
Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti
Scientific Reports Open Access 07 March 2017
-
An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori
Scientific Reports Open Access 05 March 2015
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Wimmer, E.A. Nat. Rev. Genet. 4, 225–232 (2003).
Oberstein, A., Pare, A. Kaplan, L. & Small, S. Nat. Methods 2, 583–585 (2005).
Groth, A.C., Fish, M., Nusse, R. & Calos, M.P. Genetics 166, 1775–1782 (2004).
Rong, Y.S. & Golic, K.G. Science 288, 2013–2018 (2000).
Venken, K.J.T. & Bellen, H.J. Nat. Rev. Gen. 6, 167–178 (2005).
Baer, A. & Bode, J. Curr. Opin. Biotechnol. 12, 473–480 (2001).
National Research Council. Animal Biotechnology: Science-based Concerns (The National Academic Press, Washington, District of Columbia, USA, 2002).
Irvin, N. Hoddle, M.S., O'Brochta, D.A., Carey, B. & Atkinson, P.W. Proc. Natl. Acad. Sci. USA 101, 891–896 (2004).
Handler, A.M., Zimowska, G.J. & Horn, C. Nat. Biotechnol. 22, 1150–1154 (2004).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wimmer, E. Insect transgenesis by site-specific recombination. Nat Methods 2, 580–582 (2005). https://doi.org/10.1038/nmeth0805-580
Issue Date:
DOI: https://doi.org/10.1038/nmeth0805-580
This article is cited by
-
Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii
BMC Genetics (2020)
-
Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti
Scientific Reports (2017)
-
An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori
Scientific Reports (2015)
-
Less is more: strategies to remove marker genes from transgenic plants
BMC Biotechnology (2013)
-
Recombination technologies for enhanced transgene stability in bioengineered insects
Genetica (2011)