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We apologize in advance to the many researchers whose excellent papers could not be referenced, because of space limitations. The methods described in this paper are those presently used at the Pittsburgh Development Center, USA and in the Department of Surgery, Cambridge, UK. They were developed in collaboration with our many good colleagues in the HESC field and in close conjunction with the faculty of our Frontiers in Human Embryonic Stem Cells Course taught annually at the University of Pittsburgh. Also contributing to the preparation and review of this manuscript were many laboratory colleagues, particularly H. Qidwai, J. Mich-Basso and C. Redinger for editorial review.
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Schatten, G., Smith, J., Navara, C. et al. Culture of human embryonic stem cells. Nat Methods 2, 455–463 (2005) doi:10.1038/nmeth0605-455
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