By sampling a two-dimensional diffraction pattern on a spherical detector, three-dimensional structure determination of single molecules should be possible from a single measurement.
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References
Raines, K.S. et al. Three-dimensional structure determination from a single view. Nature advance online publication (16 December 2009).
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Doerr, A. One-shot structure determination. Nat Methods 7, 96–97 (2010). https://doi.org/10.1038/nmeth0210-96a
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DOI: https://doi.org/10.1038/nmeth0210-96a