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Acknowledgements
J.-S.K. is supported by a grant from the Institute for Basic Science (IBS-R021-D1).
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J.-S.K. is a cofounder of and holds stocks in ToolGen, Inc., a company focused on therapeutic genome editing. All the other authors declare no competing financial interests.
Integrated supplementary information
Supplementary Figure 1
(a) Sample-specific SNVs in all pairwise combinations F03, F05, and FVB (WT) (A vs B means variants present in B and not in A). All three aligned sequencing data representing WT (SRR5450998), F03 (SRR5450997), and F05 (SRR5450996) were retrieved and analyzed using Strelka and Mutect. All SNVs were filtered using dbSNP 143 and mouse genome project v5. (b,c) Venn diagrams showing homozygous (blue) and heterozygous (red) SNVs (b) and indels (c) found in the wild-type control mouse (FVB) and the two gene-edited mice (F03 and F05). Variants were identified with Platypus (v. 1.0.8) with multi-sample callingmode with default parameters; variants with coverage lower than 15 in any of the samples, and multi-allelic variants are not considered.
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Kim, ST., Park, J., Kim, D. et al. Response to “Unexpected mutations after CRISPR–Cas9 editing in vivo”. Nat Methods 15, 239–240 (2018). https://doi.org/10.1038/nmeth.4554
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DOI: https://doi.org/10.1038/nmeth.4554
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