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References
Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–243, 1, 243 (2015).
Kim, D., Kim, S., Kim, S., Park, J. & Kim, J.S. Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq. Genome Res. 26, 406–415 (2016).
Kim, D. et al. Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells. Nat. Biotechnol. 34, 863–868 (2016).
Aken, B.L. et al. Ensembl 2017. Nucleic Acids Res. 45, D635–D642 (2017)
Acknowledgements
We thank M. Schlesner and R. Eils for helpful discussion. This work was supported by the Institute for Basic Science grant no. IBS-R021-D1 to J.-S.K. and Next Generation BioGreen 21 Program grant no. PJ01119201 and Korea Healthcare technology R&D Project grant no. HI16C1012 to S.B.
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Park, J., Childs, L., Kim, D. et al. Digenome-seq web tool for profiling CRISPR specificity. Nat Methods 14, 548–549 (2017). https://doi.org/10.1038/nmeth.4262
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DOI: https://doi.org/10.1038/nmeth.4262
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