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Stratifying tissue heterogeneity with scalable single-cell assays

Three methods dramatically improve the scale of single-cell genomic sequencing and structural assays, allowing for the hierarchical partitioning of cell populations within a tissue.

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Figure 1: Routes for scaling up single-cell assays.

Marina Corral Spence/Springer Nature


  1. Zahn, H. et al. Nat. Methods 14, 167–173 (2017).

    Article  CAS  Google Scholar 

  2. Vitak, S.A. et al. Nat. Methods 14, 302–308 (2017).

    Article  CAS  Google Scholar 

  3. Ramani, V. et al. Nat. Methods 14, 263–266 (2017).

    Article  CAS  Google Scholar 

  4. Marcy, Y. et al. PLoS Genet. 3, e155 (2007).

    Article  Google Scholar 

  5. Adey, A. et al. Genome Biol. 11, R119 (2010).

    Article  CAS  Google Scholar 

  6. Amini, S. et al. Nat. Genet. 46, 1343–1349 (2014).

    Article  CAS  Google Scholar 

  7. Adey, A. et al. Genome Res. 24, 2041–2049 (2014).

    Article  CAS  Google Scholar 

  8. Cusanovich, D.A. et al. Science 348, 910–914 (2015).

    Article  CAS  Google Scholar 

  9. Nagano, T. et al. Nature 502, 59–64 (2013).

    Article  CAS  Google Scholar 

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Correspondence to Kun Zhang.

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Zhang, K. Stratifying tissue heterogeneity with scalable single-cell assays. Nat Methods 14, 238–239 (2017).

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