Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Overcoming obstacles in localization microscopy

Lattice light-sheet-PAINT brings localization microscopy to large volumes, and SuReSim enables ground truth modeling of super-resolution experiments.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Localization microscopy beyond the coverslip.

References

  1. Hell, S.W. Nat. Methods 6, 24–32 (2009).

    Article  CAS  Google Scholar 

  2. Xu, K., Zhong, G. & Zhuang, X. Science 339, 452–456 (2013).

    Article  CAS  Google Scholar 

  3. Legant, W.R. et al. Nat. Methods 13, 359–365 (2016).

    Article  Google Scholar 

  4. Venkataramani, V., Herrmannsdörfer, F., Heilemann, M. & Kuner, T. Nat. Methods 13, 319–321 (2016).

    Article  Google Scholar 

  5. Axelrod, D. J. Cell Biol. 89, 141–145 (1981).

    Article  CAS  Google Scholar 

  6. Tokunaga, M., Imamoto, N. & Sakata-Sogawa, K. Nat. Methods 5, 159–161 (2008).

    Article  CAS  Google Scholar 

  7. Cella Zanacchi, F. et al. Nat. Methods 8, 1047–1049 (2011).

    Article  Google Scholar 

  8. Sharonov, A. & Hochstrasser, R.M. Proc. Natl. Acad. Sci. USA 103, 18911–18916 (2006).

    Article  CAS  Google Scholar 

  9. Chen, B.C. et al. Science 346, 1257998 (2014).

    Article  Google Scholar 

  10. Jungmann, R. et al. Nano Lett. 10, 4756–4761 (2010).

    Article  CAS  Google Scholar 

  11. Jungmann, R. et al. Nat. Methods 11, 313–318 (2014).

    Article  CAS  Google Scholar 

  12. Kiuchi, T., Higuchi, M., Takamura, A., Maruoka, M. & Watanabe, N. Nat. Methods 12, 743–746 (2015).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ralf Jungmann.

Ethics declarations

Competing interests

R.J. is cofounder of Ultivue, Inc., a startup company with interest in commercializing DNA-PAINT super-resolution technology.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jungmann, R. Overcoming obstacles in localization microscopy. Nat Methods 13, 301–302 (2016). https://doi.org/10.1038/nmeth.3813

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.3813

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing