Obtaining deep proteomic coverage by mass spectrometry has typically been a long, labor-intensive task involving extensive fractionation and lengthy instrument analysis times. Hebert et al. now report an approach for comprehensively analyzing the yeast proteome in just over 1 hour. Their highly optimized protocol includes improved sample preparation and chromatographic separation and, most importantly, the latest-generation Orbitrap Fusion mass spectrometer technology. This instrument possesses a high tandem-mass-spectral acquisition speed and a control environment with multiple independent processing units. The protocol was highly reproducible and yielded an average identification of nearly 4,000 proteins, about 90% of the expressed yeast proteome. This achievement shows that mass spectrometry technology can be an efficient, routine tool for profiling proteomes: the authors estimate that the expressed human proteome could be analyzed in a few hours with their approach.
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A proteome in an hour. Nat Methods 10, 1147 (2013). https://doi.org/10.1038/nmeth.2739
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DOI: https://doi.org/10.1038/nmeth.2739
