Developing tools to detect the rare mutations found in tumors demands a lot of data and rigorous benchmarking.
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References
Cibulskis, K. et al. Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples. Nat. Biotechnol. 31, 213–219 (2013).
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Nawy, T. Tumors have their differences. Nat Methods 10, 282–283 (2013). https://doi.org/10.1038/nmeth.2426
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DOI: https://doi.org/10.1038/nmeth.2426