A combination of mutagenesis in a pathogen and RNA interference in its host cell reveals the role of non-essential bacterial genes.
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References
O'Connor T.J. et al. Aggravating genetic interactions allow a solution to redundancy in a bacterial pathogen. Science 338, 1440–1444 (2012).
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Rusk, N. How parasitic pathogens do it. Nat Methods 10, 102–103 (2013). https://doi.org/10.1038/nmeth.2355
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DOI: https://doi.org/10.1038/nmeth.2355