Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Omnidirectional microscopy

In toto imaging of living embryos has now become much faster. Light-sheet illumination and fluorescence detection with four objective lenses provide complete coverage of large samples in a snap.

This is a preview of subscription content

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Microscopes with multiple objective lenses provide complementary views of the same sample for in toto imaging and real-time developmental biology studies.


  1. Weber, M. & Huisken, J. Curr. Opin. Genet. Dev. 21, 566–572 (2011).

    CAS  Article  Google Scholar 

  2. Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Science 305, 1007–1009 (2004).

    CAS  Article  Google Scholar 

  3. Krzic, U., Gunther, S., Saunders, T.E., Streichan, S.J. & Hufnagel, L. Nat. Methods 9, 755–763 (2012).

    Article  Google Scholar 

  4. Tomer, R., Khairy, K., Amat, F. & Keller, P.J. Nat. Methods 9, 730–733 (2012).

    Article  Google Scholar 

  5. Swoger, J., Huisken, J. & Stelzer, E.H.K. Opt. Lett. 28, 1654–1656 (2003).

    Article  Google Scholar 

  6. Huisken, J. & Stainier, D.Y.R. Development 136, 1963–1975 (2009).

    CAS  Article  Google Scholar 

  7. Huisken, J. & Stainier, D.Y.R. Opt. Lett. 32, 2608–2610 (2007).

    Article  Google Scholar 

  8. Verveer, P.J. et al. Nat. Methods 4, 311–313 (2007).

    CAS  Article  Google Scholar 

  9. Preibisch, S., Saalfeld, S., Schindelin, J. & Tomancak, P. Nat. Methods 7, 418–419 (2010).

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Jan Huisken.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Weber, M., Huisken, J. Omnidirectional microscopy. Nat Methods 9, 656–657 (2012).

Download citation

  • Published:

  • Issue Date:

  • DOI:

Further reading


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing