Urban, N.T. et al. Biophys. J. 101, 1277–1284 (2011).
To study synapse function in a physiological setting, it is necessary to image deep in the brain. Urban et al. describe adaptation to a stimulated emission depletion (STED) super-resolution to facilitate actin imaging inside synapses 120 micrometers below the tissue surface. They equipped their STED microscope with a high-numerical-aperture glycerol-immersion objective lens with a correction collar, which greatly decreased aberrations resulting from light scattering.
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Deep imaging with STED. Nat Methods 8, 897 (2011). https://doi.org/10.1038/nmeth.1757
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DOI: https://doi.org/10.1038/nmeth.1757