Abstract
We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag–based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables large-scale, multiplexed quantitative proteomics using isobaric tagging.
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Acknowledgements
We thank A.J. Bureta for figure illustrations, A. Williams for proofreading, A. Ledvina and D. Bailey for assistance with instrument firmware code modifications, S. Hubler for theoretical calculations regarding yeast and human peptides, J. Brumbaugh and J. Thomson for culturing the human cells, and J. Syka, J. Schwartz, V. Zabrouskov, J. Griep-Raming and D. Nolting for helpful discussions. This work was supported by US National Institutes of Health grant R01 GM080148 to J.J.C. D.H.P. acknowledges support from an National Institutes of Health Genomic Sciences Training Program (5T32HG002760).
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C.D.W. designed and performed research, and wrote the paper; M.V.L., A.S.H. and G.C.M. designed and performed research; D.H.P. designed research; M.S.W. and J.J.C. designed research and wrote the paper.
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Two patent applications, in part related to this manuscript, are pending: US 13/086638 (C.D.W., D.H.P. and J.J.C. are the inventors) and US 61/471461 (J.J.C. and M.S.W. are the inventors).
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Wenger, C., Lee, M., Hebert, A. et al. Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging. Nat Methods 8, 933–935 (2011). https://doi.org/10.1038/nmeth.1716
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DOI: https://doi.org/10.1038/nmeth.1716
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