Brief Communication | Published:

Induced pluripotent stem cells from highly endangered species

Nature Methods volume 8, pages 829831 (2011) | Download Citation

Abstract

For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent extinction. We report generation of induced pluripotent stem cells (iPSCs) from two endangered species: a primate, the drill, Mandrillus leucophaeus and the nearly extinct northern white rhinoceros, Ceratotherium simum cottoni. iPSCs may eventually facilitate reintroduction of genetic material into breeding populations.

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Acknowledgements

I.F.B.-N. was supported by an unrestricted gift from the Esther O'Keefe Foundation, and S.C.M. was supported by an internship from the California Institute for Regenerative Medicine (TB1-01186). The research was supported by the O'Keefe Foundation and the Millipore Foundation. We thank our collaborator, M. Parast, for the histopathological examination of the teratomas, I. Slavin, X. Liao and other members of the Loring lab for helpful discussions and assistance, and G. Ben-Nun and D. Barker for support.

Author information

Affiliations

  1. Center for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.

    • Inbar Friedrich Ben-Nun
    • , Susanne C Montague
    • , Ha T Tran
    • , Ibon Garitaonandia
    • , Trevor R Leonardo
    • , Yu-Chieh Wang
    • , Louise C Laurent
    •  & Jeanne F Loring
  2. San Diego Zoo Institute for Conservation Research, Escondido, California, USA.

    • Marlys L Houck
    • , Suellen J Charter
    •  & Oliver A Ryder
  3. Department of Reproductive Medicine, University of California, San Diego, California, USA.

    • Louise C Laurent
    •  & Jeanne F Loring

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Contributions

J.F.L., O.A.R. and I.F.B.-N. conceived the study. I.F.B.-N. designed and performed the experiments. S.C.M. assisted with tissue-culture work. O.A.R., M.L.H. and S.J.C. generated the fibroblast cell lines and karyotyped the cells. H.T.T., I.G. and T.R.L. assisted with teratoma formation. Y.-C.W. performed glycomic profiling. L.C.L. contributed to experiment design. I.F.B.-N. and J.F.L. wrote the manuscript with input from all authors.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Jeanne F Loring.

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    Supplementary Text and Figures

    Supplementary Figures 1–11, Supplementary Table 1

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DOI

https://doi.org/10.1038/nmeth.1706

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