Current extracellular multisite recordings suffer from low signal-to-noise ratio, limiting the monitoring to action potentials, and preclude detection of subthreshold synaptic potentials. Here we report an approach to induce Aplysia californica neurons to actively engulf protruding microelectrodes, providing 'in-cell recordings' of subthreshold synaptic and action potentials with signal-to-noise ratio that matches that of conventional intracellular recordings. Implementation of this approach may open new vistas in neuroscience and biomedical applications.
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This work was supported by the “Brain Storm” project (EU P7 215486 STREP). Parts of the study were carried out at the Charles E. Smith and ProfessorElkes Laboratory for Collaborative Research in Psychobiology. The fabrication of the gold spines electrode was carried at the Harvey M. Kruger Family center for Nanoscience and Nanotechnology. We thank N. Mazurski for expertise in device fabrication. M.E.S. is the Levi DeViali Professor in neurobiology. A.H. was partially supported by a scholarship from The Israel Council for Higher Education.
The authors declare no competing financial interests.
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Hai, A., Shappir, J. & Spira, M. In-cell recordings by extracellular microelectrodes. Nat Methods 7, 200–202 (2010). https://doi.org/10.1038/nmeth.1420
Nature Communications (2022)
Nano-Micro Letters (2021)
Nature Biomedical Engineering (2020)
Nature Communications (2020)