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Unusual selection on the KIR3DL1/S1 natural killer cell receptor in Africans

Abstract

Interactions of killer cell immunoglobulin-like receptors (KIRs) with major histocompatibility complex (MHC) class I ligands diversify natural killer cell responses to infection. By analyzing sequence variation in diverse human populations, we show that the KIR3DL1/S1 locus encodes two lineages of polymorphic inhibitory KIR3DL1 allotypes that recognize Bw4 epitopes of protein">HLA-A and HLA-B and one lineage of conserved activating KIR3DS1 allotypes, also implicated in Bw4 recognition. Balancing selection has maintained these three lineages for over 3 million years. Variation was selected at D1 and D2 domain residues that contact HLA class I and at two sites on D0, the domain that enhances the binding of KIR3D to HLA class I. HLA-B variants that gained Bw4 through interallelic microconversion are also products of selection. A worldwide comparison uncovers unusual KIR3DL1/S1 evolution in modern sub-Saharan Africans. Balancing selection is weak and confined to D0, KIR3DS1 is rare and KIR3DL1 allotypes with similar binding sites predominate. Natural killer cells express the dominant KIR3DL1 at a high frequency and with high surface density, providing strong responses to cells perturbed in Bw4 expression.

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Figure 1: Human 3DL1/S1 is highly polymorphic.
Figure 2: The KIR3DL1/S1 locus comprises three ancient lineages of diverse inhibitory and conserved activating receptors.
Figure 3: Positive selection on the D1 and D2 domains of 3DL1/S1 focuses on residues predicted to bind MHC class I.
Figure 4: Worldwide distribution of KIR3DL1/S1 uncovers many alleles in Africa.
Figure 5: Natural selection for HLA-Bw4 frequency and variation.
Figure 6: Functional diversity of 3DL1/S1 is lowest in Africans and localizes to D0.

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Acknowledgements

We thank all donors, phlebotomists and colleagues involved in sample preparation; D. Koechlein, M. Lawson and members of N. Pourmand's laboratory for technical advice; and M. Carrington, S. Cooper and D. Middleton's laboratories for further samples. M.G. is a Howard Hughes graduate scholar and D.K. a Boehringer Ingelheim Fonds graduate scholar. S.S. is a Wellcome Trust Training fellow, and P.J.N. is a Lymphoma Research Foundation fellow. This work was supported grants to P.P. from the US National Institutes of Health (AI17892, AI24258 and AI64520) and by the Leukaemia and Lymphoma Society of the USA.

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Experiments were designed by P.J.N. and M.R. and performed by P.J.N., K.G., M.G., D.K., D.R. and D.B. Subjects were recruited, samples collected and DNA extracted by C.V.F.C., D.C., Y.-H.C., C.C., G.S.-D., P.A.F., K.H., G.K., K.A.K., Z.L., N.M., J.M., M.H.P., R.M.P., D.D.R., M.-Y.S., H.A.F.S., S.S., R.W.V., D.H.V., D.T. and E.M.R. Additional resources were provided by R.W.D. and E.M.R. Analysis was performed by P.J.N. and L.A.-R. The paper was written by P.J.N., L.A.-R. and P.P.

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Correspondence to Paul J Norman or Peter Parham.

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Norman, P., Abi-Rached, L., Gendzekhadze, K. et al. Unusual selection on the KIR3DL1/S1 natural killer cell receptor in Africans. Nat Genet 39, 1092–1099 (2007). https://doi.org/10.1038/ng2111

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