We have created a global map of the effects of polymorphism on gene expression in 400 children from families recruited through a proband with asthma. We genotyped 408,273 SNPs and identified expression quantitative trait loci from measurements of 54,675 transcripts representing 20,599 genes in Epstein-Barr virus–transformed lymphoblastoid cell lines. We found that 15,084 transcripts (28%) representing 6,660 genes had narrow-sense heritabilities (H2) > 0.3. We executed genome-wide association scans for these traits and found peak lod scores between 3.68 and 59.1. The most highly heritable traits were markedly enriched in Gene Ontology descriptors for response to unfolded protein (chaperonins and heat shock proteins), regulation of progression through the cell cycle, RNA processing, DNA repair, immune responses and apoptosis. SNPs that regulate expression of these genes are candidates in the study of degenerative diseases, malignancy, infection and inflammation. We have created a downloadable database to facilitate use of our findings in the mapping of complex disease loci.
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Gene Expression Omnibus
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The study was funded by the Wellcome Trust, the Medical Research Council, the French Ministry of Higher Education and Research and the US National Institutes of Health (the National Human Genome Research Institute and the National Heart, Lung and Blood Institute).
Supplementary Figures 1–2 (PDF 149 kb)
Transcripts and SNPs with H2>0.3 and LOD scores for association>6. (XLS 2671 kb)
Summary of sequential search for independent associations of SNPs with individual transcripts (XLS 55 kb)
Potential Master Regulators (XLS 456 kb)
Gene Ontology (Biological Process) analyses for most highly heritable traits (XLS 60 kb)
Lists of genes in the three most significant GO-BP classes (XLS 1059 kb)
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Dixon, A., Liang, L., Moffatt, M. et al. A genome-wide association study of global gene expression. Nat Genet 39, 1202–1207 (2007). https://doi.org/10.1038/ng2109
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