A screen of the complete protein kinase gene family identifies diverse patterns of somatic mutations in human breast cancer

Article metrics


We examined the coding sequence of 518 protein kinases, 1.3 Mb of DNA per sample, in 25 breast cancers. In many tumors, we detected no somatic mutations. But a few had numerous somatic mutations with distinctive patterns indicative of either a mutator phenotype or a past exposure.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Figure 1: Breast cancer mutational spectra.


  1. 1

    Manning, G., Whyte, D.B., Martinez, R., Hunter, T. & Sudarsanam, S. Science 298, 1912–1934 (2002).

  2. 2

    Huang, M.E., Rio, A.G., Nicolas, A. & Kolodner, R.D. Proc. Natl. Acad. Sci. USA. 100, 11529–11534 (2003).

  3. 3

    Xiao, W., Fontanie, T., Bawa, S. & Kohalmi, L. Mutat. Res. 431, 155–165 (1999).

  4. 4

    Wagner, E. & Lykke-Andersen, J. J. Cell Sci. 115, 3033–3038 (2002).

  5. 5

    Persons, D.L., Borelli, K.A. & Hsu, P.H. Mod. Pathol. 10, 720–727 (1997).

  6. 6

    Wang, T.L. et al. Proc. Natl. Acad. Sci. USA 99, 3076–3080 (2002).

  7. 7

    Olivier, M. et al. Hum. Mutat. 19, 607–614 (2002).

Download references

Author information

Correspondence to Michael R Stratton.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Table 1

Mutation data for the primary and secondary screen (PDF 47 kb)

Supplementary Table 2

Genes analysed in the primary and secondary screens (XLS 66 kb)

Supplementary Table 3

Samples. (PDF 40 kb)

Supplementary Table 4

Coding strand. (PDF 52 kb)

Supplementary Table 5

Pyrimidine strand. (PDF 51 kb)

Supplementary Methods (PDF 78 kb)

Rights and permissions

Reprints and Permissions

About this article

Further reading