A radiation hybrid map of mouse genes

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Abstract

A comprehensive gene-based map of a genome is a powerful tool for genetic studies and is especially useful for the positional cloning and positional candidate approaches. The availability of gene maps for multiple organisms provides the foundation for detailed conserved-orthology maps showing the correspondence between conserved genomic segments. These maps make it possible to use cross-species information in gene hunts and shed light on the evolutionary forces that shape the genome. Here we report a radiation hybrid map of mouse genes, a combined project of the Whitehead Institute/Massachusetts Institute of Technology Center for Genome Research, the Medical Research Council UK Mouse Genome Centre, and the National Center for Biotechnology Information. The map contains 11,109 genes, screened against the T31 RH panel1 and positioned relative to a reference map containing 2,280 mouse genetic markers2. It includes 3,658 genes homologous to the human genome sequence3 and provides a framework for overlaying the human genome sequence to the mouse and for sequencing the mouse genome.

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Figure 1: Steps of the fluorescence polarization (FP)–based detection system following single-base extension (SBE)9 of a PCR product for high-throughput radiation-hybrid (RH) mapping.
Figure 2: Graphic of mouse radiation hybrid map segments overlaid on the human genome sequence.

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Acknowledgements

We thank G. Farino and V. Frazzoni for technical assistance. T.J.H. is a recipient of a Clinician-Scientist award from the Canadian Institutes of Health Research. This research was supported by the US National Institutes of Health (HG01806), the UK Medical Research Council and EC contract QLG2-CT-1999-00988.

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Correspondence to Thomas J. Hudson.

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Hudson, T., Church, D., Greenaway, S. et al. A radiation hybrid map of mouse genes. Nat Genet 29, 201–205 (2001) doi:10.1038/ng1001-201

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