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High resolution localization of recombination hot spots using sperm typing

Nature Genetics volume 7, pages 420–424 (1994)Cite this article

Abstract

We have applied sperm DNA typing to determine the distribution of crossover events within a one megabase region of the short arm of human chromosome 4 near the locus for Huntington disease. A total of 29 recombinants were detected among 602 sperm typed after whole genome amplification. These recombinants were typed for seven polymorphic markers. The 280 kilobase D4S10–D4S126 interval was found to undergo recombination at a 6–9–fold greater rate per unit of physical distance than the adjacent 720 kb D4S126–D4S127 interval. Sperm typing has the potential to dissect mammalian recombination hot spots to the point where DNA sequence analysis may reveal the molecular basis for hyperrecombination.

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Author notes

  1. Rene Hubert

    Present address: Medical Genetics Program, Cedars-Sinai Medical Center, Los Angeles, California, 90048, USA

Authors and Affiliations

  1. Molecular Biology Program, University of Southern California, Los Angeles, California, 90089-1340, USA

    Rene Hubert & Norman Arnheim

  2. Neurogenetics Program, Massachusetts General Hospital, Boston, Massachusetts, 02114, USA

    Marcy MacDonald & James Gusella

Authors
  1. Rene Hubert
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  2. Marcy MacDonald
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  3. James Gusella
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  4. Norman Arnheim
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Hubert, R., MacDonald, M., Gusella, J. et al. High resolution localization of recombination hot spots using sperm typing. Nat Genet 7, 420–424 (1994). https://doi.org/10.1038/ng0794-420

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