An important aspect of multi-step tumorigenesis is the mutational activation of genes of the RAS family, particularly in sporadic cancers of the pancreas, colon, lung and myeloid system1. RAS genes encode small GTP-binding proteins that affect gene expression in a global way by acting as major switches in signal transduction processes, coupling extracellular signals with transcription factors2,3,4. Oncogenic forms of RAS are locked in their active state and transduce signals essential for transformation, angiogenesis, invasion and metastasis via downstream pathways involving the RAF/MEK/ERK cascade of cytoplasmic kinases, the small GTP-binding proteins RAC and RHO, phosphatidylinositol 3-kinase and others5,6. We have used subtractive suppression hybridization (SSH), a PCR-based cDNA subtraction technique7, to contrast differential gene expression profiles in immortalized, non-tumorigenic rat embryo fibroblasts and in HRAS- transformed cells. Sequence and expression analysis of more than 1,200 subtracted cDNA fragments revealed transcriptional stimulation or repression of 104 ESTs, 45 novel sequences and 244 known genes in HRAS- transformed cells compared with normal cells. Furthermore, we identified common and distinct targets in cells transformed by mutant HRAS, KRAS and NRAS, as well as 61 putative target genes controlled by the RAF/MEK/ERK pathway in reverted cells treated with the MEK-specific inhibitor PD 98059.
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We thank S. Petermann, J. Keil and K. Skultety for technical assistance; R. Brinckmann for sequencing; C. Schlüns for computing; F. Leenders and F. Theuring for help with phospho-image analysis; and M. Dietel for generous support. Our work was supported by Deutsche Krebshilfe (grant 10-332-Schä I to R.S.), Krebsliga des Kantons Zürich (grant to R.S.), Studienstiftung des Deutschen Volkes and Forschungsförderung der Charité (to J.Z.), and Berliner Krebsgesellschaft (grant to R.S.).
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