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Natural variation at Strubbelig Receptor Kinase 3 drives immune-triggered incompatibilities between Arabidopsis thaliana accessions

Nature Genetics volume 42, pages 1135–1139 (2010)Cite this article

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Abstract

Accumulation of genetic incompatibilities within species can lead to reproductive isolation and, potentially, speciation. In this study, we show that allelic variation at SRF3 (Strubbelig Receptor Family 3), encoding a receptor-like kinase, conditions the occurrence of incompatibility between Arabidopsis thaliana accessions. The geographical distribution of SRF3 alleles reveals that allelic forms causing epistatic incompatibility with a Landsberg erecta allele at the RPP1 resistance locus are present in A. thaliana accessions in central Asia. Incompatible SRF3 alleles condition for an enhanced early immune response to pathogens as compared to the resistance-dampening effect of compatible SRF3 forms in isogenic backgrounds. Variation in disease susceptibility suggests a basis for the molecular patterns of a recent selective sweep detected at the SRF3 locus in central Asian populations.

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Figure 1: SRF3 allelic forms and world-wide distribution of incompatible alleles.
Figure 2: Genetic structure and SRF3 allelic diversity.
Figure 3: Immune responses in compatible and incompatible lines.
Figure 4: Normalized Fay and Wu's H statistic across the SRF3 genomic region in central Asian (red) and north European (blue) accessions.

Accession codes

Accessions

ArrayExpress

NCBI Reference Sequence

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Acknowledgements

We thank C. Alonso-Blanco, V. le Corre, M.H. Hoffmann, K. Schmid, O.A. Rognli and O. Loudet for providing seed materials. We thank B. Huettel for microarray hybridizations. This work was funded by Deutsche Forschungsgemeinschaft SFB 680 grants (to M.R., J.E.P. and J.d.M.). The authors also acknowledge the Max Planck Society and an International Max Planck Research School fellowship to A.V.G.

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Author notes

  1. Ana V García & Jane E Parker

    Present address: Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, Germany. Present addresses: Institut Jean-Pierre Bourgin, UMR1318 INRA-AgroParisTech, Institut National de la Recherche Agronomique (INRA) Centre de Versailles-Grignon, Route de St-Cyr (RD10), Versailles Cedex, France (M.R.) and Unité de Recherche en Génomique Végétale, INRA-Centre National de la Recherche Scientifique-Université Evry Val d'Essonne (CNRS-UEVE), Evry CEdex, France (A.V.G.).,

Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Max Planck Institute for Plant Breeding Research, Cologne, Germany

    Rubén Alcázar, Ilkka Kronholm, Juliette de Meaux, Maarten Koornneef & Matthieu Reymond

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  1. Rubén Alcázar
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Contributions

R.A., M.K., J.E.P. and M.R. conceived the study. R.A. performed most of the experimental work with contributions from A.V.G. in the pathogen infection assays. I.K. and J.d.M. performed the computer analysis and interpretation of Fay and Wu's Hn statistics. M.K. provided accessions and European F2 populations. J.E.P. provided materials for immune analyses. M.R. performed all statistical analyses. All authors analyzed the data. R.A., J.E.P and M.R. wrote the paper with contributions from all authors.

Corresponding author

Correspondence to Matthieu Reymond.

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Supplementary Figures 1–11 and Supplementary Tables 1–6 (PDF 1990 kb)

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Alcázar, R., García, A., Kronholm, I. et al. Natural variation at Strubbelig Receptor Kinase 3 drives immune-triggered incompatibilities between Arabidopsis thaliana accessions. Nat Genet 42, 1135–1139 (2010). https://doi.org/10.1038/ng.704

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