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Lgr5 marks cycling, yet long-lived, hair follicle stem cells

Nature Genetics volume 40, pages 12911299 (2008) | Download Citation


In mouse hair follicles, a group of quiescent cells in the bulge is believed to have stem cell activity. Lgr5, a marker of intestinal stem cells, is expressed in actively cycling cells in the bulge and secondary germ of telogen hair follicles and in the lower outer root sheath of anagen hair follicles. Here we show that Lgr5+ cells comprise an actively proliferating and multipotent stem cell population able to give rise to new hair follicles and maintain all cell lineages of the hair follicle over long periods of time. Lgr5+ progeny repopulate other stem cell compartments in the hair follicle, supporting the existence of a stem or progenitor cell hierarchy. By marking Lgr5+ cells during trafficking through the lower outer root sheath, we show that these cells retain stem cell properties and contribute to hair follicle growth during the next anagen. Expression analysis suggests involvement of autocrine Hedgehog signaling in maintaining the Lgr5+ stem cell population.

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We thank D. Roop and W. Buitrago for help with setting up the keratinocyte transplantation assay; Å.-L. Dackland for help with cell sorting; Å. Bergström and I. Sur for contributions during work with animals and preparation of samples; and M. van den Born, M. Cozijnsen and H. Begthel for help with histological procedures. This work was supported by the Swedish Cancer Society (to R.T.), the Swedish Research Council (to R.T.), the National Institutes of Health (AR47898 and U01CA105491 to R.T.), Genmab BV (to H.C.), Koninklijke Nederlandse Akademie van Wetenschappen (to H.C.), Koningin Wilhelmina Fonds (to H.C.), the Louis Jeantet Foundation (to H.C.), an International Agency for Research on Cancer Research Training Fellowship (to V.J.), a Marie-Curie Intra-European Fellowship (to V.J.) and a fellowship from the Wenner-Gren Foundation (M.K.).

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Author notes

    • Viljar Jaks
    • , Nick Barker
    •  & Maria Kasper

    These authors contributed equally to this work.


  1. Karolinska Institutet, Department of Biosciences and Nutrition, Novum, SE-141 57 Huddinge, Sweden.

    • Viljar Jaks
    • , Maria Kasper
    •  & Rune Toftgård
  2. University of Tartu, Institute of Molecular and Cell Biology and Estonian Biocentre, Riia 23, 51010 Tartu, Estonia.

    • Viljar Jaks
  3. Hubrecht Institute, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.

    • Nick Barker
    • , Johan H van Es
    • , Hugo J Snippert
    •  & Hans Clevers


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Corresponding authors

Correspondence to Hans Clevers or Rune Toftgård.

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