DURING productive infection of human cells with types 2 or 5 adenovirus a number of proteins specific for the infected cells, but not found in the intact virion, can be detected by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS)1, 2. None of these proteins, however, has been isolated in a native state and therefore their functions remain obscure. The observation that replicating adenovirus DNA contained extensive single stranded regions (refs 3, 4, and U. Petterson, personal communication) led us to look for proteins specific for infected cells that bind only to single stranded DNA and may be involved directly in DNA replication. Such a class of proteins, originally described by Alberts and Frey5, has now been found in a number of prokaryotic systems5–7 as well as in mammalian cells8, 9, and they are known, at least in the case of T4 gene 32 protein, to be required for DNA replication and genetic recombination5. To eliminate the large excess of adenovirus coat proteins, synthesised in human cells, which are known to bind to DNA10, we used African green monkey cells as hosts. In these cells viral DNA synthesis occurs at a normal rate but late viral capsid proteins are not produced or are synthesised in very small amounts11.
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VAN DER VLIET, P., LEVINE, A. DNA-binding Proteins specific for Cells infected by Adenovirus. Nature New Biology 246, 170–174 (1973). https://doi.org/10.1038/newbio246170a0
Protocol of electron microscope in situ nucleic acid hybridization for the exclusive detection of double-stranded DNA sequences in cells containing large amounts of homologous single-stranded DNA and RNA sequences: Application to adenovirus type 5 infected Hela cells
Microscopy Research and Technique (1993)
A zinc-binding motif located between amino acids 273 and 286 in the adenovirus DNA-binding protein is necessary for ssDNA binding