Abstract
Using sarcoplasmic reticulum (SR) membranes, stacked and orientated by centrifugation, Dupont, Harrison and Hasselbach1 demonstrated that it was possible to record Bragg reflections to 15 Å−1 resolution. The phases of the reflections and the electron density profiles were obtained to a resolution of 30 Å. In an attempt to label the calcium pump protein with heavy atoms very dramatic changes were observed in the X-ray diffraction pattern when organo-mereurials or other SH reagents were bound to the ATPase. These changes seem to have been due to large structural modifications of the membrane.
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References
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DUPONT, Y., HASSELBACH, W. Structural Changes in Sarcoplasmic Reticulum Membrane induced by SH Reagents. Nature New Biology 246, 41–44 (1973). https://doi.org/10.1038/newbio246041a0
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DOI: https://doi.org/10.1038/newbio246041a0
