Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Isolation of Feather Keratin mRNA and its Translation in a Rabbit Reticulocyte Cell-free System

Nature New Biology volume 246, pages 33–36 (1973)Cite this article

Abstract

12S keratin mRNA isolated from duck embryo feathers is translated in rabbit reticulocyte lysate with a high degree of fidelity, without any requirement for additional components from feather tissue.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Bell, E., and Thathachari, Y. T., J. Cell Biol., 16, 215 (1963).

    Article  CAS  Google Scholar 

  2. Kemp, D. J., Rogers, G. E., and Dyer, P. Y., cell Biol. (submitted).

  3. Humphreys, S., Penman, S., and Bell, E., Biochem. biophys. Res. Commun., 17, 618 (1965).

    Article  Google Scholar 

  4. Yatvin, M. B., Science, N.Y., 151, 1001 (1966).

    Article  CAS  Google Scholar 

  5. Fraser, R. D. B., MacRae, T. P., and Rogers, G. E., Keratins: Their Composition, Structure and Biosynthesis (Thomas, Springfield, Illinois, 1972).

    Google Scholar 

  6. Kemp, D. J., and Rogers, G. E., Biochemistry, 11, 969 (1972).

    Article  CAS  Google Scholar 

  7. O'Donnell, I. J., Aust. J. biol. Sci., 26, 415 (1973).

    Article  CAS  Google Scholar 

  8. Kitos, P. A., Saxon, G., and Amos, H., Biochem. biophys. Res. Commun., 47, 1426 (1972).

    Article  CAS  Google Scholar 

  9. Williamson, R., Morrison, M., Lanyon, G., Eason, R., and Paul, J., Biochemistry, 10, 3014 (1971).

    Article  CAS  Google Scholar 

  10. Lee, S. Y., Mendecki, J., and Brawerman, G., Proc. natn. Acad. Sci. U.S.A., 68, 1331 (1971).

    Article  CAS  Google Scholar 

  11. Atlas of Protein Sequence and Structure (edit, by Dayhoff, M. O.), 4, D42, D54 (National Biomedical Research Foundation, Silver Spring, Maryland, 1969).

  12. Evans, M. J., and Lingrel, J. B., Biochemistry, 8, 829 (1969).

    Article  CAS  Google Scholar 

  13. Adamson, S. D., Herbert, E., and Godchaux, W., III, Archs biochem. Biophys., 125, 671 (1968).

    Article  CAS  Google Scholar 

  14. Hunt, T., Vanderhoff, G., and London, I. M., J. molec. Biol., 66, 471 (1972).

    Article  CAS  Google Scholar 

  15. Gross, M., and Rabinovitz, M., Biochim. biophys. Acta, 299, 472 (1973).

    Article  CAS  Google Scholar 

  16. Swank, R. T., and Munkres, K. D., Anal Biochem., 39, 462 (1972).

    Article  Google Scholar 

  17. Ward, S., Wilson, D. L., and Gillian, J. J., Anal. Biochem., 38, 90 (1970).

    Article  CAS  Google Scholar 

  18. Strous, G. J. A. M., Berns, A. J. M., van Westreenan, H., and Bloemendal, H., Eur. J. Biochem., 30, 48 (1972).

    Article  CAS  Google Scholar 

  19. Berns, A. J. M., Van Rraaikamp, M., Bloemendal, H., and Lane, C. D., Proc. natn. Acad. Sci. U.S.A., 69, 1606 (1972).

    Article  CAS  Google Scholar 

  20. Lim, L., and Canellakis, E. S., Nature, 227, 710 (1970).

    Article  CAS  Google Scholar 

  21. Edmonds, M., Vaughan, M. H., jun., and Nakazato, H., Proc. natn. Acad. Sci. U.S.A., 68, 1336 (1971).

    Article  CAS  Google Scholar 

  22. Darnell, J. E., Wall, R., and Tushinski, R. J., Proc. natn. Acad. Sci. U.S.A., 68, 1321 (1971).

    Article  CAS  Google Scholar 

  23. Firtel, R. A., Jacobson, A., and Lodish, H. F., Nature new Biol., 239, 225 (1972).

    Article  CAS  Google Scholar 

  24. Sheldon, R., Jurale, C., and Kates, J., Proc. natn. Acad. Sci. U.S.A., 69, 417 (1972).

    Article  CAS  Google Scholar 

  25. Pemberton, R. E., and Baglioni, C., J. molec. Biol., 65, 531 (1972).

    Article  CAS  Google Scholar 

  26. Burr, H., and Lingrel, J. B., Nature new Biol., 233, 41 (1971).

    Article  CAS  Google Scholar 

  27. Molloy, G. R., Sporn, M. B., Kelley, D. E., and Perry, R. P., Biochemistry, 11, 3256 (1972).

    Article  CAS  Google Scholar 

  28. Nakazato, H., Kopp, D. W., and Edmonds, M., J. biol. Chem., 248, 1472 (1973).

    CAS  PubMed  Google Scholar 

  29. McLaughlin, C. S., Warner, J. R., Edmonds, M., Nakazato, H., and Vaughan, M. H., J. biol. Chem., 248, 1466 (1973).

    CAS  PubMed  Google Scholar 

  30. Schutz, G., Beato, M., and Feigelson, P., Biochem. biophys. Res. Commun., 49, 680 (1972).

    Article  CAS  Google Scholar 

  31. DeLarco, J., and Guroff, G., Biochem. biophys. Res. Commun., 50, 486 (1973).

    Article  CAS  Google Scholar 

  32. Lockard, R. E., and Lingrel, J. B., Biochem. biophys. Res. Commun., 37, 204 (1969).

    Article  CAS  Google Scholar 

  33. Stavnezer, J., and Huang, R. C. C., Nature new Biol., 230, 172 (1971).

    Article  CAS  Google Scholar 

  34. Rhoads, R. E., McKnight, G. S., and Schimke, R. T., J. biol. Chem., 246, 7407 (1971).

    CAS  PubMed  Google Scholar 

  35. Berns, A. J. M., Strous, G. J. A. M., and Bloemendal, H., Nature new Biol., 236, 7 (1972).

    Article  CAS  Google Scholar 

  36. Rosenfeld, G. C., Comstock, J. P., Means, A. R., and O'Malley, B. W., Biochem. biophys. Res. Commun., 46, 1695 (1972).

    Article  CAS  Google Scholar 

  37. Gallwitz, D., and Breindl, M., Biochem. biophys. Res. Commun., 47, 1106 (1972).

    Article  CAS  Google Scholar 

  38. Pemberton, R. E., Housman, D., Lodish, H. F., and Baglioni, C., Nature new Biol., 235, 99 (1972).

    Article  CAS  Google Scholar 

  39. Mach, B., Faust, C., and Vassalli, P., Proc. natn. Acad. Sci. U.S.A., 70, 451 (1973).

    Article  CAS  Google Scholar 

  40. Gurdon, J. B., Lane, C. D., Woodland, H. R., and Marbaix, G., Nature new Biol., 233, 177 (1971).

    Article  CAS  Google Scholar 

  41. Heywood, S. M., Proc. natn. Acad. Sci. U.S.A., 67, 1782 (1970).

    Article  CAS  Google Scholar 

  42. Wigle, D. T., and Smith, A. E., Nature new Biol., 242, 136 (1973).

    Article  CAS  Google Scholar 

  43. Schreier, M. H., and Staehelin, T., J. molec. Biol., 73, 329 (1973).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Adelaide, Adelaide, South Australia, 5001

    GEOFFREY A. PARTINGTON, DAVID J. KEMP & GEORGE E. ROGERS

Authors
  1. GEOFFREY A. PARTINGTON
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. DAVID J. KEMP
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. GEORGE E. ROGERS
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

PARTINGTON, G., KEMP, D. & ROGERS, G. Isolation of Feather Keratin mRNA and its Translation in a Rabbit Reticulocyte Cell-free System. Nature New Biology 246, 33–36 (1973). https://doi.org/10.1038/newbio246033a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio246033a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing