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Infectious Herpesvirus DNA

Nature New Biology volume 245, pages 265–266 (1973)Cite this article

Abstract

RABBIT kidney and fibroblast cells, after exposure to the isolated DNA of herpes simplex virus in the presence of DEAE-dextran, have been shown to develop a cytopathic effect1. Enhancement of plaque formation with DNA using DEAE-dextran has been described2,3 but a new technique has been recently reported which gives a 100-fold increase in efficiency over the DEAE-dextran method for human adenovirus DNA4. Here we report that using this technique the DNA of herpes simplex virus type I (HSV-I) can be shown to be infectious and plaques can be obtained on BSC-1 cells (African green monkey kidney cells).

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References

  1. Lando, D., and Rhyhiner, M. L., C.r. hebd. Séanc. Acad. Sci. Paris, 269, 527 (1969).

    CAS  Google Scholar 

  2. Pagano, J. S., and Vaheri, A., Arch. ges. Virusforsch., 17, 456 (1965).

    Article  CAS  Google Scholar 

  3. Warden, D., and Thorne, H. V., J. gen. Virol., 3, 371 (1968).

    Article  CAS  Google Scholar 

  4. Graham, F. L., and van der Eb, A. J., Virology, 52, 456 (1973).

    Article  CAS  Google Scholar 

  5. Dulbecco, R., and Vogt, M., J. expl. Med., 99, 167 (1954).

    Article  CAS  Google Scholar 

  6. Weil, R., Virology, 14, 46 (1961).

    Article  CAS  Google Scholar 

  7. Burton, K., Biochem. J., 62, 315 (1956).

    Article  CAS  Google Scholar 

  8. Brown, M., Ritchie, D. A., and Subak-Sharpe, J. H., J. gen. Virol., 18, 329 (1973).

    Article  CAS  Google Scholar 

  9. Wilkie, N. M., J. gen. Virol, (in the press).

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Author information

Authors and Affiliations

  1. Laboratory for Physiological Chemistry, State University of Leiden, Wassenaarseweg, 62, Leiden

    F. L. GRAHAM

  2. Medical Biological Laboratory, TNO, Rijswijk

    G. VELDHUISEN

  3. The Institute of Virology, The University of Glasgow, Church Street, Glasgow, G11 5JR

    N. M. WILKIE

Authors
  1. F. L. GRAHAM
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  2. G. VELDHUISEN
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  3. N. M. WILKIE
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GRAHAM, F., VELDHUISEN, G. & WILKIE, N. Infectious Herpesvirus DNA. Nature New Biology 245, 265–266 (1973). https://doi.org/10.1038/newbio245265a0

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  • DOI: https://doi.org/10.1038/newbio245265a0

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