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Distribution of Lactate Dehydrogenase Isozymes in Rat Liver Cells

Nature New Biology volume 245, pages 239–240 (1973)Cite this article

Abstract

MAMMALIAN L-lactate dehydrogenase (LDH) (EC. 1.1.1.27.) is a tetrameric molecule consisting of two types of subunits (H and M) which by random combination give five possible isozymes (LDH-1 (H4) to LDH-5 (M5))1. In tissues consisting of different cell types such as the liver, a particular isozyme distribution may be an expression of the pattern in all cell types of the organ, or, alternatively, different cell types may have different isozyme patterns. In studying the isozyme distribution as a function of hormone administration2, carcinogenesis3, metabolic changes4 or differentiation5 it is of obvious importance to know the contribution from the different cell types to the total isozyme pattern. The mammalian liver consists primarily of hepatocytes (parenchymal cells) and reticuloendothelial cells (Kupffer cells)6. As much as 35% of the cells are Kupffer cells, representing 5 to 10% of the liver mass6,7. To obtain information about the isozyme distribution in the different liver cells we have tried to separate Kupffer and parenchymal cells after enzymatic dispersion of rat liver.

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References

  1. Pesche, A., Fondy, T. P., Stoltenbach, F., Castillo, F., and Kaplan, N. O., J. biol. Chem., 242, 2151 (1967).

    Google Scholar 

  2. Goodfriend, T. L., and Kaplan, N. O., J. biol. Chem., 239, 130 (1964).

    CAS  PubMed  Google Scholar 

  3. Shonk, C. E., and Boxer, G. E., Methods in Cancer Research, 11, 581, (Academic Press, New York, 1967).

    Google Scholar 

  4. Kaplan, N. O., Everse, J., and Admiraal, J., Ann. N.Y. Acad. Sci., 151, 400 (1968).

    Article  CAS  Google Scholar 

  5. Ikeda, T., Okamoto, N., and Satow, Y., Hiroshima J. med. Sci., 18, 13 (1969).

    CAS  PubMed  Google Scholar 

  6. Benacerraf, B., The Liver, 2, 37, (Academic Press, New York, 1963).

    Google Scholar 

  7. Daoust, R., Liver Function, 3 (American Institute of Biological Sciences, 1958).

  8. Berry, M. N., and Friend, D. S., J. Cell. Biol, 43, 506 (1969).

    Article  CAS  Google Scholar 

  9. Berg, T., Boman, D., and Seglen, P. O., Expl Cell Res., 72, 571 (1972).

    Article  CAS  Google Scholar 

  10. Seglen, P. O., Expl. Cell Res., 74, 450 (1972).

    Article  CAS  Google Scholar 

  11. Sweat, F. W., and Hupka, A., Biochem. biophys. Res. Commun., 44, 1436 (1971).

    Article  CAS  Google Scholar 

  12. Mills, D. N., and Zucker-Franklin, D., Am. J. Path., 54, 147 (1969).

    CAS  PubMed  Google Scholar 

  13. Dietz, A. A., and Lubrano, T., Analyt. Biochem., 20, 246 (1967).

    Article  CAS  Google Scholar 

  14. Dietz, A. A., Lubrano, T., and Rubinstein, H. M., Clinica chim. Acta, 27, 225 (1970).

    Article  CAS  Google Scholar 

  15. Fritz, P. J., Morrison, W. J., White, E. L., and Vesell, E. S., Analyt. Biochem., 36, 443 (1970).

    Article  CAS  Google Scholar 

  16. Lowry, O. H., Rosebrough, N. J., Fair, A. L., and Randall, R. J., J. biol. Chem., 193, 265 (1951).

    CAS  Google Scholar 

  17. Sigma tech. Bull., 500 (September 1967).

  18. Fritz, P. J., Vesell, E. S., White, E. L., and Pruitt, K. M., Proc. natn. Acad. Sci. U.S.A., 62, 558 (1969).

    Article  CAS  Google Scholar 

  19. Kaplan, N. O., and Everse, J., Enzyme Regulation, 10, 323, (Pergamon Press, New York, 1972).

    Article  CAS  Google Scholar 

  20. Karnovsky, M. L., Semin. Hematol., 5, 156 (1968).

    CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Zoophysiology, University of Oslo, Blindern, Oslo, 3

    TROND BERG

  2. Institute of Medical Biology, University of Tromsø, Tromsø

    ARNOLDUS SCHYTTE BLIX

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  1. TROND BERG
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  2. ARNOLDUS SCHYTTE BLIX
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BERG, T., BLIX, A. Distribution of Lactate Dehydrogenase Isozymes in Rat Liver Cells. Nature New Biology 245, 239–240 (1973). https://doi.org/10.1038/newbio245239a0

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