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Platelet Aggregation induced by Arachidonic Acid is accompanied by Release of Potential Inflammatory Mediators distinct from PGE2 and PGF2

Nature New Biology volume 244, pages 114–116 (1973)Cite this article

Abstract

ARACHIDONIC acid (AA) is converted into prostaglandins (PG) E2 and F2α by PG synthetase contained in mammalian tissues1,2. A material showing vasocontracting activity and called rabbit aorta contracting substance (RCS)3 has been suggested to be the cyclic endoperoxide intermediate formed during the biosynthesis of these prostaglandins4,5. The participation of RCS in inflammation has been proposed as its biosynthesis, like that of PG, is inhibited by anti-inflammatory drugs (AID)5–7. Platelets synthesize PG when reacted with collagen or thrombin8,9 or directly with AA10. Collagen-induced platelet aggregation is inhibited by AID11 and contact with collagen by damaged vessels is believed to be the initial stimulus for platelet aggregation in vivo12. No connexion has been proposed as yet between platelet aggregation, synthesis of PG and their common inhibition by AID. We have now demonstrated that AA induces platelet aggregation with generation of RCS and PG. As both responses were hindered by AID, it is suggested that PG synthetase interferes with platelet function other than through PG. An intermediate such as RCS may play the central role. Human or rabbit platelet rich plasma (hPRP or rPRP) and other blood elements were obtained as indicated in Fig. 1 and aggregation was studied turbidimetrically13 at room temperature.

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References

  1. Bergstrom, S., Danielsson, H., and Samuelsson, B., Biochim. Biophys. Acta, 90, 207 (1954).

    Article  Google Scholar 

  2. Van Dorp, D. A., Beerthuis, R. K., Nugteren, D. H., and Vonkeman, H., Biochim. Biophys. Acta, 90, 204 (1964).

    Article  PubMed  Google Scholar 

  3. Piper, P. J., and Vane, J. R., Nature, 223, 29 (1969).

    Article  CAS  PubMed  Google Scholar 

  4. Gryglewski, R., and Vane, J. R., Brit. J. Pharmacol., 43, 420P (1971).

    Google Scholar 

  5. Gryglewski, R., and Vane, J. R., Brit. J. Pharmacol., 45, 37 (1972).

    Article  CAS  Google Scholar 

  6. Vargaftig, B. B., and Dao Hai, N., Pharmacology, 6, 99 (1971).

    Article  CAS  PubMed  Google Scholar 

  7. Vargaftig, B. B., and Dao Hai, N., in Vasopeptides (edit. by Back, N., and Sicuteri, F.), 155 (Plenum, New York, 1971).

    Google Scholar 

  8. Smith, J. B., and Willis, A. L., Nature New Biology, 231, 235 (1971).

    Article  CAS  PubMed  Google Scholar 

  9. Smith, J. B., Kocsis, J. J., Ingerman, C., and Silver, M. J., in Abstr. of the Fifth Internat. Cong. Pharmacol., 217 (San Francisco, 1972).

    Google Scholar 

  10. Clausen, J., and Srivastava, K. C., Lipids, 7, 246 (1972).

    Article  CAS  PubMed  Google Scholar 

  11. Mustard, J. F., and Packham, M. A., Pharmacol. Rev., 22, 97 (1970).

    CAS  PubMed  Google Scholar 

  12. Packham, M. A., Nishizawa, E., and Mustard, J. F., Biochem. Pharmacol., 17, Suppl., 171 (1968).

  13. Born, G. V. R., Nature, 104, 927 (1962).

    Article  Google Scholar 

  14. Vane, J. R., Brit. J. Pharmacol., 23, 360 (1964).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Vane, J. R., Brit. J. Pharmacol., 12, 344 (1957).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Regoli, D., and Vane, J. R., Brit. J. Pharmacol., 23, 351 (1964).

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Vargaftig, B. B., and Dao Hai, N., in Advances in the Biosciences, 9, 74 (Pergamon Press, Vieweg, 1972).

    Google Scholar 

  18. Berry, P. A., thesis, University of London (1966).

  19. Rosenberg, F. J., Gimber-Phillips, P. E., Grobewski, G. E., Davison, C., Phillips, D. K., Goralnick, S. J., and Cahill, E. D., J. Pharmacol. Exp. Therap., 170, 410 (1971).

    Google Scholar 

  20. O'Brien, J. R., Lancet, i, 894 (1968).

  21. Willis, A. L., J. Pharm. Pharmacol., 21, 126 (1969).

    Article  CAS  PubMed  Google Scholar 

  22. Kloeze, J., Biochim. Biophys. Acta, 187, 285 (1969).

    Article  CAS  PubMed  Google Scholar 

  23. MacGee, J., in Methods of Enzymatic Analysis (edit. by Bergmeyer, H. U.), 41 (Academic Press, New York, 1965).

    Google Scholar 

  24. Ferreira, S. H., and Vargaftig, B. B., Proc. Brit. Microcirculation Soc., Biorheology (in the press).

  25. Gryglewski, R., and Vane, J. R., Brit. J. Pharmacol., 46, 449 (1972).

    Article  CAS  Google Scholar 

  26. Okuma, M., Steiner, M., and Baldini, M., J. Lab. Clin. Med., 77, 728 (1971).

    CAS  PubMed  Google Scholar 

  27. Haslam, R. J., Nature, 202, 765 (1964).

    Article  CAS  PubMed  Google Scholar 

  28. Flower, R., Gryglewski, R., Herbaczymska-Cedro, K., and Vane, J. R., Nature, 238, 104 (1972).

    CAS  Google Scholar 

  29. Vargaftig, B. B., Miranda, E. P., and Lacoume, B., Nature, 222, 883 (1969).

    Article  CAS  PubMed  Google Scholar 

  30. Marcus, A. J., William, H. L., and Saner, L. B., J. Lipid Res., 10, 108 (1969).

    CAS  PubMed  Google Scholar 

  31. Astrup, T., Symposium: le rôle de la paroi artérielle dans l'Athérogénèse, CNRS, 535 (1967).

Download references

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  1. B. B. VARGAFTIG

    Present address: Merrell International Research Centre, 16, Rue d'Ankara, 67000, Strasbourg, France

Authors and Affiliations

  1. Orgamon R.D. S.A.R.L., Eragny-sur-Epte 60590, Serifontaine

    B. B. VARGAFTIG & P. ZIRINIS

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VARGAFTIG, B., ZIRINIS, P. Platelet Aggregation induced by Arachidonic Acid is accompanied by Release of Potential Inflammatory Mediators distinct from PGE2 and PGF2. Nature New Biology 244, 114–116 (1973). https://doi.org/10.1038/newbio244114a0

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