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Dissociation of Responses to Phytohaemagglutinin and Adult Allogeneic Lymphocytes in Human Foetal Lymphoid Tissues

Nature New Biology volume 241, pages 279–281 (1973)Cite this article

Abstract

IN vitro reactivity to phytohaemagglutinin (PHA) and to allogeneic lymphocytes in the one way mixed lymphocyte reaction (MLR) is associated with lymphocytes which are thymus derived1–4 and usually correlates well with cell mediated immune competence in vivo5–8. That these responses may be the property of separate cell populations has been suggested by observations that (a) a fraction of rat thymocytes separated on an albumin density gradient reacted in an MLR but not to PHA, while other fractions were responsive in both tests9; (b) human lymphocytes treated with trypsin and Vibrio cholera extract react in an MLR but have a depressed response to PHA10; and (c) rare reports of PHA-responsive MLR-nonresponsive lymphocytes in immune deficient states11. We wished to determine whether MLR and PHA responses are possessed by the same or different populations of lymphoid cells during human foetal development. Results indicate that the MLR is acquired earlier in development and is independent of the PHA response, and that foetal liver is the site of MLR-responsive PHA-nonresponsive cells.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of California, San Francisco, California, 94122

    M. C. CARR

  2. Section of Hematology and Immunology, Department of Medicine, University of California, San Francisco, California, 94122

    D. P. STITES & H. H. FUDENBERG

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  1. M. C. CARR
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  2. D. P. STITES
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  3. H. H. FUDENBERG
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CARR, M., STITES, D. & FUDENBERG, H. Dissociation of Responses to Phytohaemagglutinin and Adult Allogeneic Lymphocytes in Human Foetal Lymphoid Tissues. Nature New Biology 241, 279–281 (1973). https://doi.org/10.1038/newbio241279a0

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